Study on mammalian spermatozoa freeze-dried to withstand high temperature storage
| Project/Area Number |
25430188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservation of biological resources
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | フリーズドライ / 精子 / 高温保存 / 室温保存 / 染色体 / ＤＮＡ / 受精卵 / コメットアッセイ / 水酸化カリウム / 染色体異常 / フリーズドライ精子 / 発生 / アルカリ性 / 水素イオン濃度 / DNAダメージ / 高温耐性獲得 / 酸性 |
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| Outline of Final Research Achievements |
Chromosomes (DNAs) of freeze-dried mouse spermatozoa could withstand high tempeature storage for short term (3 days to 1 week, 40 or 50°C) or long term (1 to 8 months, 25°C), when the spermatozoa were freeze-dried using EGTA/Tris-HCl-bufferred solution (ETBS) adjusted its pH values to 8.0 to 8.4, or using sodium-free ETBS containing potassium ions.
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Report
(4 results)
Research Products
(3 results)
