| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
In this study, we aimed to elucidate molecular mechanisms of evolutionally conserved control of cyclin B1 mRNA translation to understand the nature of translational machineries by which special and temporal translation is regulated. First, we demonstrated that binding of Pumilio1 to cyclin B1 mRNA is essential for the RNA granule formation, which was found in vertebrate oocytes, and that this Pumilio1-mediated RNA granule formation is important for specially and temporally regulated translational activation of the mRNA. Then, we identified IMP3 protein as a novel factor binding to cycle B1 mRNA, providing novel insight into the translational regulation of the mRNA. Furthermore, we generated transgenic animals visualizing dynamics of actin filaments and obtained results supporting the notion that assembly and depolymerization of actin filaments are involved in the translational regulation.
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