Identification of the gene responsible for the spermatogenesis defects of a C. elegans mutant
| Project/Area Number |
25440002
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
Ushida Chisato 弘前大学, 農学生命科学部, 准教授 (50250593)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
武藤 あきら 弘前大学, 農学生命科学部, 研究員 (80034635)
姫野 俵太 弘前大学, 農学生命科学部, 教授 (80208785)
|
|---|
| Project Period (FY) |
2013-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
|---|
| Keywords | リボソーム / U8 snoRNA / 精子形成 / 胚発生 / 受精卵 / 卵殻 / rRNA前駆体 / ncRNA / 受精異常 / 卵形成 / 減数分裂 / 核小体RNA / 線虫 |
|---|
| Outline of Final Research Achievements |
MT16939 is a Caenorhabditis elegans mutant which lacks a region of chromosome IV. There are parts of protein coding genes of unknown function and a small nucleolar RNA (snoRNA). MT16939 shows spermatogenesis defects, embryonic development defects and an egg-shape change. To understand the relationships between the genes and the phenotype alterations, nine novel strains of C. elegans were prepared by microinjection or MosSCI system. The spermatogenesis defects and the embryonic development defects could not be recovered by the introduction of each gene. It is possible that two or three of these genes are required for the recoveries. The change of egg-shape was rescued by the introduction of snoRNA gene.
|
Report
(5 results)
Research Products
(10 results)
