| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
C. merolae nuclear genome contains number of intronic tRNA genes and circularly permuted tRNA genes. Precursors derived from both tRNA genes contain the BHB motif, which is the primal recognition site for the tRNA-splicing enzyme. Terefore, both tRNAs are considered to be processed by tRNA-splicing machinery and be evlutionaly correlated each other. This study showed that the permuted tRNA can be developed from tandemly repeated intronic tRNA genes in C. merolae cells. We also found that variously disrupted tRNA genes can be expressed in C. merolae cells probably because of the noncanonical tRNA maturation pathway.
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