Contribution of tRNA-splicing machinery in development of permuted and intronic tRNA genes.
Project/Area Number |
25440003
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | Chiba University |
Principal Investigator |
Akiko Soma 千葉大学, 園芸学研究科, 助教 (70350329)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | RNAプロセシング / 翻訳 / tRNA / 遺伝子進化 / RNA / 遺伝子 / 逆転tRNA遺伝子 / C. merolae / RNA purocessing / タンデムtRNA遺伝子 |
Outline of Final Research Achievements |
C. merolae nuclear genome contains number of intronic tRNA genes and circularly permuted tRNA genes. Precursors derived from both tRNA genes contain the BHB motif, which is the primal recognition site for the tRNA-splicing enzyme. Terefore, both tRNAs are considered to be processed by tRNA-splicing machinery and be evlutionaly correlated each other. This study showed that the permuted tRNA can be developed from tandemly repeated intronic tRNA genes in C. merolae cells. We also found that variously disrupted tRNA genes can be expressed in C. merolae cells probably because of the noncanonical tRNA maturation pathway.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Identification of highly-disrupted tRNAs in nuclear genome of the red alga, Cyanidioschyzon merolae 10D.2013
Author(s)
Akiko Soma, Junichi Sugahara, Akinori Onodera, Nozomu Yachie, Akio Kanai, Satoru Watanabe, Hirofumi Yoshikawa, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, and Yasuhiko Sekine
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 2321-2329
DOI
Related Report
Peer Reviewed
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