| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Overall objective of this study is to elucidate the molecular mechanism of translation system in mammalian mitochondria. As a result, we aim to lay the basis for the medical application, such as the design of antibiotics. Utilizing the reconstituted mitochondrial translation system, we have dissected the function of mitochondrial ICT1, a mitoribosomal protein, and yet a member of peptide release factor family. Its substrate specificity as well as the functional domain has been analyzed. We demonstrated the possible engagement of ICT1 in the translation termination at non-standard stop codons AGA and AGG of two mitochondrial ORFs MTCOI and MTND6, respectively. Structures of the mitoribosomal complexes have been determined by cryoEM reconstruction. The structures of PRE- and POST-translocation complex have been determined at high resolution of approximately 4 angstrom.
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