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Reconstitution of mammalian mitochondrial translation system and its application

Research Project

Project/Area Number 25440004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

Tomita-Takeuchi Nono  東京大学, 新領域創成科学研究科, 准教授 (80323450)

Research Collaborator Akabane Shiori  東京大学, 大学院新領域創成科学研究科, 博士課程大学院生
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords翻訳 / ミトコンドリア / 生体外タンパク質合成系 / タンパク質合成
Outline of Final Research Achievements

Overall objective of this study is to elucidate the molecular mechanism of translation system in mammalian mitochondria. As a result, we aim to lay the basis for the medical application, such as the design of antibiotics. Utilizing the reconstituted mitochondrial translation system, we have dissected the function of mitochondrial ICT1, a mitoribosomal protein, and yet a member of peptide release factor family. Its substrate specificity as well as the functional domain has been analyzed. We demonstrated the possible engagement of ICT1 in the translation termination at non-standard stop codons AGA and AGG of two mitochondrial ORFs MTCOI and MTND6, respectively. Structures of the mitoribosomal complexes have been determined by cryoEM reconstruction. The structures of PRE- and POST-translocation complex have been determined at high resolution of approximately 4 angstrom.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (14 results)

All 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] IMPB, Charite, Berlin(Germany)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Response to the Formal Letter of Z Chrzanowska-Lightowlers and RN Lightowlers regarding our article "Ribosome rescue and translation termination at non-standard stop codons by ICT1 in mammalian mitochondria"2015

    • Author(s)
      Nierhaus, KH. & Takeuchi N.
    • Journal Title

      PLoS Genet.

      Volume: 11(6) Issue: 6 Pages: e1005218-e1005218

    • DOI

      10.1371/journal.pgen.1005218

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Oxidation of translation factor EF-G transiently retards the translational elongation cycle in Escherichia coli2015

    • Author(s)
      Nagano, T., Yutthanasirikul, R., Hihara, Y., Hisabori, T., Kanamori, T., Takeuchi, N., Ueda, T. and Nishiyama, Y.
    • Journal Title

      J. Biochem.

      Volume: - Issue: 2 Pages: 165-72

    • DOI

      10.1093/jb/mvv026

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ribosome rescue and translation termination at non-standard stop codons by ICT1 in mammalian mitochondria2014

    • Author(s)
      Akabane, S., Ueda, T., Knud H. Nierhaus, KH. & Takeuchi N.
    • Journal Title

      PLOS Genetics

      Volume: 18 Issue: 9 Pages: e1004616-e1004616

    • DOI

      10.1371/journal.pgen.1004616

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes2013

    • Author(s)
      Kotani, T.
    • Journal Title

      Nucleic acids research

      Volume: 2013 Issue: 6 Pages: 3713-3722

    • DOI

      10.1093/nar/gkt079

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear Respiratory Factor 2beta (NRF-2beta) Recruits NRF-2alpha to the Nucleus by Binding to Importin-alpha:beta via an Unusual Monopartite-Type Nuclear Localization Signal2013

    • Author(s)
      Hayashi, R.
    • Journal Title

      Journal of molecular biology

      Volume: 425 Issue: 18 Pages: 3536-3548

    • DOI

      10.1016/j.jmb.2013.07.007

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 翻訳伸長制御のメカニズムとその意義2013

    • Author(s)
      富田(竹内)野乃、上田卓也
    • Journal Title

      実験医学増刊号『生命分子を統合するRNA』

      Volume: 31 Pages: 1108-1116

    • Related Report
      2013 Research-status Report
  • [Presentation] Unique translation termination in mammalian mitochondria2015

    • Author(s)
      Nono Tomita-Takeuchi
    • Organizer
      Molecular Mitochondria
    • Place of Presentation
      Helsinki, Finland
    • Year and Date
      2015-10-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 真核細胞におけるnon-canonicalな翻訳制御因子Stm1の機能解析2014

    • Author(s)
      林光、上田卓也、伊藤耕一、梶昭、竹内野乃
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Characterization of eukarytic translation initiation factor 5A from Saccharomyces cerevisiae.2013

    • Author(s)
      Satoshi Nishimura, Hikari Hayashi, Tetsuya Kotani, Takuya Ueda, Koichi Ito, Nono Tomita-Takeuchi
    • Organizer
      2013 RIboclub annual meeting
    • Place of Presentation
      ケベック(カナダ)
    • Related Report
      2013 Research-status Report
  • [Presentation] The Regulation of elongation Phase2013

    • Author(s)
      Hikari Hayashi 1, Satoshi Nishimura 1, Tetsuya Kotani 1, Syun Utsunomiya 1, Masanari Ishibashi 1, Takuya Ueda, Koichi Ito, Akira Kaji, Nono Tomita-Takeuchi
    • Organizer
      2013 RIboclub annual meeting
    • Place of Presentation
      ケベック(カナダ)
    • Related Report
      2013 Research-status Report
  • [Presentation] 哺乳類ミトコンドリアにおける異常停止したリボゾーム複合体の再生機構2013

    • Author(s)
      赤羽しおり、小谷哲也、入江遼平、上田卓也、竹内野乃
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県県民文化会館(日本)
    • Related Report
      2013 Research-status Report
  • [Presentation] Protein Synthesis System of Mammalian Mitochondria

    • Author(s)
      Nono Takeuchi
    • Organizer
      ASMRM2013
    • Place of Presentation
      ソウル(韓国)
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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