Genomic instability by Topoisomerase 1 induced by AID-dependent RNA editing
Project/Area Number |
25440007
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Molecular biology
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Research Institution | Kyoto University |
Principal Investigator |
Kobayashi Maki 京都大学, 医学(系)研究科(研究院), 准教授 (20400690)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | トポイソメラーゼ1 / AID / ゲノム不安定性 / 免疫グロブリン遺伝子 / 遺伝子不安定性 / Topoisomerase1 / miRNA / Ago2 |
Outline of Final Research Achievements |
In order to identify the responsive site in Topoisomerase 1 (Top1) mRNA to AID, Top1 3'UTR knockout cells were generated from CH12 B lymphoma cells. Unexpectedly class switch recombination in these knockout cells was not abolished supposing that coding region of Top1 mRNA is the target of AID's regulation. On the other hand, Top1's involvement in DNA cleavages in immunoglobulin (Ig) gene was further clarified by the proteasome inhibitor and the novel Top1 inhibitors. Additionally by Top1-GFP fusion protein and trapping by GFP, it is elucidated that FACT enrolls as an adopter molecule between Top1 and H3K4me3, modified histone with help of BRG1.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] LSD1/KDM1A promotes hematopoietic commitment of hemangioblasts through downregulation of Etv2.2015
Author(s)
Takeuchi, M., Fuse, Y., Watanabe, M., Andrea, C.S., Takeuchi, M., Nakajima, H., Ohashi, K., Kaneko, H., Kobayashi-Osaki, M., Yamamoto, M. and Kobayashi, M.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 112
Issue: 45
Pages: 13922-13927
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Activation-induced cytidine deaminase is dispensable for virus-mediated liver and skin tumor development in mouse models.2014
Author(s)
Nguyen T, Xu J, Chikuma S, Hiai H, Kinoshita K, Moriya K, Koike K, Marcuzzi GP, Pfister H, Honjo T, Kobayashi M.
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Journal Title
Int Immunol
Volume: 26
Issue: 7
Pages: 397-406
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Activation-induced cytidine deaminase is dispensable for virus-mediated liver and skin tumor development in mouse models in press2014
Author(s)
Tung Nguyen,Jianliang Xu,Shunsuke Chikuma,Hiroshi Hiai,Kazuo Kinoshita,Kyoji Moriya,Kazuhiko Koike,Gian Paolo Marcuzzi, Herbert Pfister, Tasuku Honjo, and Maki Kobayashi
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] GATA factor switching from GATA2 to GATA1 contributes to erythroid differentiation2013
Author(s)
Suzuki M, Kobayashi-Osaki M, Tsutsumi S, Pan X, Ohmori S, Takai J, Moriguchi T, Ohneda O, Ohneda K, Shimizu R, Kanki Y, Kodama T, Aburatani H, Yamamoto M
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Journal Title
Genes Cells
Volume: 18
Issue: 11
Pages: 921-933
DOI
Related Report
Peer Reviewed
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