Development of novel method of Zero Mode Waveguides for characterization of DNA binding proteins
| Project/Area Number |
25440008
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
HAN YONG-WOON 京都大学, 物質－細胞統合システム拠点, 助教 (50566297)
|
|---|
| Research Collaborator |
IWASA Takuma 京都大学, 大学院生命科学研究科, 大学院生
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 蛍光１分子イメージング / DNA結合タンパク質 |
|---|
| Outline of Final Research Achievements |
Zero Mode Waveguides (ZMWs) consist of nanosized holes in an aluminum film. Thus, ZMWs enable us the visualization of a single fluorescent molecule under micromolar order of the molecules. We characterized E. coli RuvB motor protein, which promotes Holliday junction DNA branch migration, and SRA, hemi-methyl CpG binding domain. We observed fluorescently labeled RuvBs binding to a RuvA-Holliday junction under various nucleotide conditions and our data suggested that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation
|
Report
(4 results)
Research Products
(25 results)
-
-
[Journal Article] ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.2015
Author(s)
Oomoto, I., Suzuki-Hirano, A., Umeshima, H., Han, YW., Yanagisawa, H., Carlton, P., Harada, Y., Kengaku, M., Okamoto, A., Shimogori, T. and Wang, DO.
-
Journal Title
Nucleic Acids Res.
Volume: 43(19)
Issue: 19
Pages: e126-e126
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation.2015
Author(s)
Iwasa, T., Han, Y. W., Hiramatsu, R., Yokota, H., Nakao, K., Yokokawa, R., Ono, T., Harada, Y.
-
Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 18177-18177
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Construction and Characterization of Cy3- or Cy5-Conjugated Hairpin Pyrrole / Imidazole Polyamide Binding to DNA in the Nucleosome2014
Author(s)
Han, Y-W.; Tsunaka, Y.; Yokota, H.; Matsumoto, T.; Kashiwazaki, G.; Morinaga, H.; Hashiya, K.; Bando, T.; Sugiyama, H.; Harada, Y.
-
Journal Title
Biomater. Sci.
Volume: 2
Issue: 3
Pages: 297-307
DOI
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] In vivo fluorescent ATP imaging of Drosophila melanogaster and Caenorhabditis elegans by using a genetically encoded fluorescent ATP biosensor optimized for low temperatures.2013
Author(s)
Tsuyama, Taiichi; Kishikawa, Jun-ichi; Han, Yong-Woon; Harada, Yoshie; Tsubouchi, Asako; Noji, Hiroyuki; Kakizuka, Akira; Yokoyama, Ken; Uemura, Tadashi; Imamura, Hiromi.
-
Journal Title
Analytical Chemistry
Volume: 85(16)
Issue: 16
Pages: 7889-7896
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
