| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Heat shock response is regulated mainly at the level of transcription by heat shock factor 1 (HSF1). However, mechanisms of transcriptional induction of heat shock gene during heat shock (HSP) are not well known yet in mammalian.
I found that HSF1 forms a complex with PARP1, which has poly (ADP-ribose) polymerase activity, and PARP13, which lacks enzymatic activity. The depletion of PARP1 or PARP13 significantly reduced the induction of HSP70 mRNA during heat shock. Furthermore, HSF1 recruits PARP1 and PARP13 on HSP70 promoter region. In response to heat shock, PARP1 and PARP13 disappeared from the promoter and only PARP1 was redistributed on HSP70 gene body. HSF1-PARP complex associates with the change of chromatin components on HSP70 promoter in unstressed condition.
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