Systematic understanding of novel anti-virus system in a cell
| Project/Area Number |
25440013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shinkai Akeo 国立研究開発法人理化学研究所, 横山構造生物学研究室, 先任研究員 (10391989)
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| Co-Investigator(Renkei-kenkyūsha) |
AGARI Yoshihiro 国立研究開発法人理化学研究所, 放射光科学総合研究センター, 特別研究員 (50469889)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | CRISPR-Cas / 獲得免疫 / 超分子複合体 / 電子顕微鏡構造 / タンパク質 / 低分子RNA / リボヌクレアーゼ / Thermus thermophilus / 国際情報交換（オランダ、アメリカ） / CRISPR-Casシステム |
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| Outline of Final Research Achievements |
CRISPR-Cas system is a bacterial adaptive immune system, which degrades foreign DNA or RNA such as that derived from virus. We selected a thermophilic bacterium, Thermus thermophilus, which has several CRISPR-Cas systems, for a model organism to study their systematic manner in a cell. We investigated structure and function of two large complexes comprising two CRISPR-Cas systems of the strain. We found that each complex was composed of several protein subunits and a small RNA molecule, and adopted a helical structure. Nucleotide sequences of the small RNA molecules are similar to each other. Both complexes degraded single-strand RNA in a similar manner. These results suggest that the two complexes have been evolved from a common ancestor, and that they comprise backup system in a cell to respond to virus infection.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] RNA-targeting by the Type III-A CRISPR-Cas complex of Thermus thermophilus.2014
Author(s)
Staals R.H.J., Zhu Y., Taylor D.W., Kornfeld J.E., Sharma K., Barendregt A., Koehorst J.J., Vlot M., Varossieau K., Neupane N., Sakamoto K., Suzuki T., Dohmae N., Yokoyama S., Schaap P.J., Urlaub H., Heck A.J.R., Nogales E., Doudna J.A., Shinkai A., van der Oost J.
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Journal Title
Molecular Cell
Volume: 56
Issue: 4
Pages: 518-530
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus2013
Author(s)
Staals, R. H. J., Agari, Y., Maki-Yonekura, S., Zhu, Y., Taylor, D. W., van Duijn, E., Barendregt, A., Vlot, M., Koehorst, J. J., Sakamoto, K., Masuda, A., Dohmae, N., Schaap, P. J., Doudna, J. A., Heck, A. J. R., Yonekura, K., van der Oost, J. and Shinkai, A.
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Journal Title
Molecular Cell
Volume: 52
Issue: 1
Pages: 135-145
DOI
Related Report
Peer Reviewed
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