The significance of the carbohydrate recognition of trypsinogen in pancreatic exocrine mechanism
| Project/Area Number |
25440016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Ochanomizu University |
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Principal Investigator |
OGAWA Haruko お茶の水女子大学, 大学院人間文化創成科学研究科, 教授 (90143700)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | トリプシノーゲン / 糖鎖認識 / 膵臓外分泌 / 酵素分泌顆粒 / 糖特異的相互作用 / 膵炎抑制 / トリプシン分解抵抗性 / 糖認識部位 / トリプシン / 糖結合部位 / 分泌顆粒 / 活性化抑制 / 細胞内輸送 |
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| Outline of Final Research Achievements |
Activation of bovine pancreatic trypsinogen (BPTG) by trypsin (BPT) was found to be inhibited by D-GalN/GalNAc at pH 5.5, the pH of secretory granules in the pancreas. Binding studies with biotinylated sugar-polymers indicated that BPTG and BPT bind to a-GalNAc, a-Man, and a-Gal better at pH 5.5 than at pH 7.5. Ultraviolet-difference spectra indicated that BPTG binding to a-GalNAc differs
substantially from BPTG binding to other sugars. The N-a-benzoyl-D,L-arginine-p-nitroanilide hydrochloride-hydrolyzing activity of BPT was only slightly affected by these sugars. The results indicate that the binding of GalNAc-containing glycoconjugates protects BPTG from autoactivation, and this may be a self-defense mechanism against intrapancreatic activation.
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Report
(3 results)
Research Products
(33 results)
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[Book] Lectins: Methods and Protocols (HIrabayashi J, Ogawa H, Kobayashi Y, Tateno H (Eds.)), Chap. 45. Comprehensive List of Lectins: Origins, Natures, and Carbohydrate Specificities2014
Author(s)
Kobayashi, Y., Tateno, H., Ogawa, H., Yamamoto, K., and Hirabayashi, J.
Publisher
Humana Press
Related Report
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