| Project/Area Number |
25440017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
Shinya Ohki 北陸先端科学技術大学院大学, ナノマテリアルテクノロジーセンター, 教授 (70250420)
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| Co-Investigator(Kenkyū-buntansha) |
MORI MASASHI 石川県立大学, 生物資源工学研究所, 准教授 (00320911)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI MAMORU 大阪大学, 蛋白質研究所, 准教授 (40280507)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 気孔 / ペプチドホルモン / 受容体 / 相互作用 / 試料調製 / 植物 / 膜タンパク質 / NMR |
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| Outline of Final Research Achievements |
Purpose of this project is exploring the interaction between peptide hormones regulating stomatal density and their receptors at atomic resolution. First of all, we had established expression systems of some domains and fragments of the receptors using E. coli and/or BY-2. Next, we had extensively searched the adequate sample solution condition for analyzing the interaction. As the result of this screening for estimating the utility of various detergents, we fortunately found a certain condition for the experiments. We successfully measured a two-dimensional NMR spectrum of the unlabeled sample in this condition. Unfortunately, the screening to find the good sample condition took very long time, so that deeper structural analyses were not performed. However, the preliminary results obtained here seem to promote the research work in near future.
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