| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The protein phosphatase Cdc14p is an antagonist of mitotic cyclin-dependent kinases and is a key regulator of late mitotic events such as chromosome segregation, spindle disassembly, and cytokinesis in the budding yeast. It has been proposed that regulated nucleocytoplasmic shuttling of Cdc14p is important for exit from mitosis. We performed structural and functional characterization of the interactions between the karyopherin Kap121p (an essential nuclear import receptor in yeast) and the C-terminal region of Cdc14p. This study identified nuclear localization signals (NLSs) in the C-terminal region of Cdc14p, in which isoleucine and lysine residues play crucial roles in the interaction with Kap121p. Based on comparison with other Kap121p-specific nuclear import cargoes, we proposed “IK-NLS” as an appropriate term to refer to the Kap121p-specific NLS.
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