| Project/Area Number |
25440022
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Ritsumeikan University (2015)
Osaka University (2013-2014) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MUKAI YUKIO 長浜バイオ大学, バイオサイエンス学部, 准教授 (60252615)
SAHARA TAKEHIKO 産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (40357166)
MORITA NAOKI 産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (60371085)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Tup1-Cyc8 / コリプレッサー / 構造と機能 / Tup1-Cyc8複合体 / 構造機能相関 / Tup-Cyc8複合体 / 構造機能解析 |
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| Outline of Final Research Achievements |
The yeast Cyc8p-Tup1p protein complex is a general transcriptional corepressor of genes involved in many physiological processes. In this study, we analyze the structure and function of Cyc8p-Tup1p protein complex using X-ray crystallography and small angle X-ray scattering. Based on the three-dimensional structure, we mutated Tup1p and characterized the Cyc8p and Tup1p function. Furthermore, we succeeded to express and purify the full-length Tup1p using cold-shock yeast expression system.
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