| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In the present study, a bioinformatics approach for identification of multiple physicochemical and structural properties associated with cell-free soluble protein expression was developed. It is based on categorical data analysis of correlations between experimentally observed expression scores and multiple features of amino acid sequences calculated/predicted by bioinformatics. Using this approach, we found that protein disorder is associated with low propensity for detectable cell-free protein expression and elevated ratio of soluble expression. These tendencies are rooted in the distinct features of intrinsically disordered regions, such as low hydrophobicity, elevated surface accessibility and high abundance of sequence motifs for proteolytic degradation. We also found that the eukaryotic PTMs, such as phosphorylation, glycosylation and acetylation, display a clear preference for occurrence in disordered regions of plant proteins, whereas methylation tends to avoid disorder.
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