X-ray crystallographic study of Bloom syndrome helicase BLM and its role in prevention of tumorigenesis.
Project/Area Number |
25440024
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Kitano Ken 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40346309)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | X線構造解析 / ブルーム症候群 / ヘリカーゼ / ホリデイジャンクション / タンパク質 / DNA / ラディキシン / マトリックスメタロプロテアーゼ / ホスホジエステラーゼ / 結晶化 / X線構造解析 |
Outline of Final Research Achievements |
Bloom syndrome is a rare cancer-predisposition disorder caused by mutations of the Bloom syndrome protein (BLM). In this study, structural and biochemical studies of human BLM helicase were performed. New information about the reaction mechanism of BLM helicase toward Holliday junction (a repair intermediate in the DNA double-stranded break repair) was obtained.
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Report
(5 results)
Research Products
(19 results)
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[Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus.2013
Author(s)
Daichi Mochizuki, Toshiaki Arai, Keita Hara, Takashi Matsumoto, Ken Kitano, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Junichi Sato, Shinji Kawasaki, Shuji Kanamaru, Fumio Arisaka, Youichi Niimura
Organizer
21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods(AUC2013)
Place of Presentation
ホテルニューアカオ, 静岡県熱海市
Related Report
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