Structural study of the novel transcription mechanism essential for root development of higher plants
Project/Area Number |
25440025
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
Hirano Yoshinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529)
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Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | X線結晶構造解析 / 分子認識 / 転写因子 / GRASファミリー / IDDファミリー / 転写制御 / 根のパターン形成 / タンパク質複合体 / タンパク質相互作用 / パターン形成 / BIRD/IDDファミリー / 植物形態形成 / 分子複合体 / 結晶構造解析 |
Outline of Final Research Achievements |
Two GRAS proteins, SHR and SCR, cooperatively direct asymmetric cell division and the patterning of root cell types by transcriptional control in conjunction with BIRD/IDD transcription factors. The crystal structures of the SHR-SCR binary and JKD-SHR-SCR ternary complexes has been determined. Each GRAS domain comprises one α/β core subdomain with an α-helical cap that mediates heterodimerization by forming an intermolecular helix bundle. The α/β core subdomain of SHR forms the BIRD binding groove, which specifically recognizes the zinc fingers of JKD. We identified a conserved SHR-binding motif in 13 BIRD/IDD transcription factors. Our results establish a structural basis for GRAS-GRAS and GRAS-BIRD interactions and provide valuable clues towards our understanding of these regulators, which are involved in plant-specific signalling networks.
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Structure of the human cereblon-DDB1- lenalidomide complex reveals basis for responsiveness to thalidomide analogs2014
Author(s)
P. Chamberlain, A. Lopez-Girona, K. Miller, G. Carmel, B. Pagarigan, B. Chie-Leon, E. Rychak, L. Corral, Y. Ren, M. Wang, M. Riley, S. Delker, T. Ito, H. Ando, T. Mori, Y. Hirano, H. Handa, T. Hakoshima, T. Daniel, B. Cathers
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Journal Title
Nat. Struct. Mol. Biol.
Volume: 21
Issue: 9
Pages: 803-809
DOI
Related Report
Peer Reviewed
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