The study on inclusive catalytic reaction mechanism of the enzymes used for rare sugar production on the basis of X-ray structures in complexes with rare sugars
| Project/Area Number |
25440028
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kagawa University |
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Principal Investigator |
Yoshida Hiromi 香川大学, 総合生命科学研究センター, 准教授 (10313305)
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| Co-Investigator(Kenkyū-buntansha) |
KAMITORI SHIGEHIRO 香川大学, 総合生命科学研究センター, 教授 (00262246)
YOSHIHARA AKIHIDE 香川大学, 希少糖研究センター, 助教 (40548765)
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| Co-Investigator(Renkei-kenkyūsha) |
IZUMORI KEN 香川大学, 農学部, 名誉教授 (80035998)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | X線結晶構造解析 / 単糖異性化酵素 / 希少糖 / L-リボースイソメラーゼ / D-タガトース 3-エピメラーゼ / 触媒反応機構 / 酵素・基質複合体 / 微小重力環境タンパク質結晶化 / Ｘ線結晶解析 / Ｌ-リボースイソメラーゼ |
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| Outline of Final Research Achievements |
X-ray structures of L-ribose isomerase from Acinetobacter sp. and Cellulomonas parahominis were determined. On the basis of the wild-type and mutant form structures in complexes with rare sugars, catalytic reaction mechanism of L-RI including ring-opening mechanism of sugar was proposed. Furthermore, mutant structure analysis supported the essentiality of tetramer formation of L-RI for its enzymatic activity.
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Report
(4 results)
Research Products
(12 results)
