Study of biosynthesis mechanism of glycosaminoglycan

Research Project

Project/Area Number	25440029
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Structural biochemistry
Research Institution	Kyushu University
Principal Investigator	Kakuta Yoshimitsu 九州大学, (連合)農学研究科(研究院), 教授 (00314360)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	結晶構造解析 / 糖鎖生合成 / 糖鎖合成 / グリコサミノグリカン / 酵素 / 糖鎖 / 蛋白質 / バイオテクノロジー / 細菌
Outline of Final Research Achievements	Microbial polysaccharides are structurally identical or very similar to mammalian GAGs. To know about biosynthesis mechanism of glycosaminoglycan,we have determined the X-ray crystal structure of the enzyme. We bacterially expressed, purified and crystallized. The X-ray crystal structure of the enzyme has now been determined. We reported the structural features of the bi-functional enzyme and have discussed their implications in understanding the catalytic mechanism.

Report

(4 results)