Study of biosynthesis mechanism of glycosaminoglycan
| Project/Area Number |
25440029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Kakuta Yoshimitsu 九州大学, (連合)農学研究科(研究院), 教授 (00314360)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 結晶構造解析 / 糖鎖生合成 / 糖鎖合成 / グリコサミノグリカン / 酵素 / 糖鎖 / 蛋白質 / バイオテクノロジー / 細菌 |
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| Outline of Final Research Achievements |
Microbial polysaccharides are structurally identical or very similar to mammalian GAGs. To know about biosynthesis mechanism of glycosaminoglycan,we have determined the X-ray crystal structure of the enzyme. We bacterially expressed, purified and crystallized. The X-ray crystal structure of the enzyme has now been determined. We reported the structural features of the bi-functional enzyme and have discussed their implications in understanding the catalytic mechanism.
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Report
(4 results)
Research Products
(4 results)
