| Project/Area Number |
25440030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto Sangyo University (2015)
Kyushu University (2013-2014) |
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Principal Investigator |
NIWA Hajime 京都産業大学, 総合生命科学部, 研究員 (30610266)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIKI Yukio 九州大学, 生命防御医学研究所, 特任教授 (70261237)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | タンパク質複合体 / 結晶構造解析 / 高速AFM / ペルオキシソーム / ミトコンドリア / AAAタンパク質 / 立体構造解析 / 蛋白質複合体 / 蛋白質相互作用 / 蛋白質 / 高速原子間力顕微鏡 |
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| Outline of Final Research Achievements |
To analysis the quality control of mitochondria outer-membrane, we have crystallized Yeast Msp1 and human homologue ATAD1. Yeast Msp1 can be almost solved the structure and revealed the atomic-model which is similar to the p97-D2 domain. Pex7p is the cytosolic receptor protein that is essential for the import of peroxisomal proteins. Newly isolated as a binding protein of Pex7p, termed P7BP2, was found to be a disk-like ring with a central hole under high-speed atomic force microscope (AFM), and implicate that P7BP2 is a novel dynein-type AAA+, whose domains are arranged into a pseudo-hexameric ring structure.
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