Structural study of the peroxisomal membrane protein specific transport sysmem in the peroxisome biogenesis

• Project/Area Number: 25440030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto Sangyo University (2015); Kyushu University (2013-2014)
• Principal Investigator: NIWA Hajime 京都産業大学, 総合生命科学部, 研究員 (30610266)
• Co-Investigator(Kenkyū-buntansha): FUJIKI Yukio 九州大学, 生命防御医学研究所, 特任教授 (70261237)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: タンパク質複合体 / 結晶構造解析 / 高速AFM / ペルオキシソーム / ミトコンドリア / AAAタンパク質 / 立体構造解析 / 蛋白質複合体 / 蛋白質相互作用 / 蛋白質 / 高速原子間力顕微鏡

Outline of Final Research Achievements

To analysis the quality control of mitochondria outer-membrane, we have crystallized Yeast Msp1 and human homologue ATAD1. Yeast Msp1 can be almost solved the structure and revealed the atomic-model which is similar to the p97-D2 domain. Pex7p is the cytosolic receptor protein that is essential for the import of peroxisomal proteins. Newly isolated as a binding protein of Pex7p, termed P7BP2, was found to be a disk-like ring with a central hole under high-speed atomic force microscope (AFM), and implicate that P7BP2 is a novel dynein-type AAA+, whose domains are arranged into a pseudo-hexameric ring structure.

Research Products

• [Journal Article] Pex11p mediates peroxisomal proliferation by promoting deformation of the lipid membrane (2015)
  • Author(s): Yoshida Y., Niwa H., Honsho M., Itoyama A., Fujiki Y.
  • Journal Title: Biol. Open Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mild reduction of plasmalogens causes rhizomelic chondrodysplasia punctata: Functional characterization of a novel mutation (2014)
  • Author(s): Noguchi, M., Honsho, M., Abe, Y., Toyama, R., Niwa, H., Sato, Y., Ghaedi, K., Rahmanifar, A., Shafeghati, Y., and *Fujiki, Y.
  • Journal Title: Journal of Human Genetics Volume: 59 Issue: 7 Pages: 387-392
  • DOI: 10.1038/jhg.2014.39
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inter-ring rotations of AAA ATPase p97 revealed by electron cryomicroscopy (2014)
  • Author(s): Heidi O. Yeung, Andreas Forster, Cecilia Bebeacua, Hajime Niwa, Caroline Ewens, Ciaran McKeown, Xiaodong Zhang and Paul S. Freemont
  • Journal Title: Open Biol Volume: 4 Issue: 3 Pages: 130142-130142
  • DOI: 10.1098/rsob.130142
  • Peer Reviewed
• [Presentation] A novel AAA ATPase interacting with Pex7 modulates plasmalogen synthesis
  • Author(s): Hajime Niwa, Yasuhiro Miyauchi-Nanri, Masanori Hosho, and Yukio Fujiki
  • Organizer: 10th International Conference on AAA+ Protein From Mecahnisms and Disease to Targets
  • Place of Presentation: Comundo Conference Centre, Neuss, Germany
• [Presentation] Structural study of peroxisomal membrane protein import
  • Author(s): Hajime Niwa
  • Organizer: IMEG seminars & Minisymposium: Recent and future advances in high-speed AFM imaging of ATP/GTP-driven molecular machinery
  • Place of Presentation: Institute of Molecular Embryology and Genetics, Kumamoto University