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Structural studies of immune responses.

Research Project

Project/Area Number 25440031
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Structural biochemistry
Research InstitutionKumamoto University

Principal Investigator

Ikemizu Shinji  熊本大学, 生命科学研究部, 准教授 (60333522)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords構造生物学
Outline of Final Research Achievements

Both of interleukin (IL) -27 and IL-23 are heterodimeric cytokines belong to IL-12 family. IL-27 consists of two subunits, p28 and Epstein-Barr virus-induced gene 3 (EBI3), binds two receptors, specific WSX-1 and gp130, a common receptor subunit shared by IL-6 family. IL-27 promotes the differentiation of type 1 T helper (Th1) cells from naive helper T cell cells. IL-27 was expressed on mammalian cells, then purified. Purified IL-27 was able to bind to specific receptor WSX-1.
IL-23 receptors consist of two subunits, specific IL-23R and IL-12Rβ1 which shares with IL-12. IL-12Rβ1 was expressed on insect cells. The purified IL-12Rβ1 was mixed with IL-23, then purified as complexes with gel chromatography. Crystallization trials of the complex have been carried out. IL-23R expression trials was carried out using E. coli, insect cells and mammalian cells. We obtained IL-23R only using mammalian cells with very low yield. IL-23R might need to coexpress with the ligand and IL-12Rβ1.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results)

  • [Journal Article] Autosomal dominant immune dysregulation syndrome in humans with CTLA-4 mutations.2014

    • Author(s)
      Schubert D, et al.
    • Journal Title

      Nature Med.

      Volume: 20 Issue: 12 Pages: 1410-1416

    • DOI

      10.1038/nm.3746

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mutants of lymphotoxin-<alpha> with augmented cytotoxic activity via TNFR1 for use in cancer therapy.2013

    • Author(s)
      Morishige, T., Narimatsu, S., Ikemizu, S., Tsunooda, S., Tsutsumi Y., Mukai, Y., Okada, N., Nakagawa, S.
    • Journal Title

      Cytokine

      Volume: 61 Pages: 578-584

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of human MTH1 with a homogeneous N-terminus.2013

    • Author(s)
      Koga Y, Inazato M, Nakamura T, Hashikawa C, Chirifu M, Michi A, Yamashita T, Toma S, Kuniyasu A, Ikemizu S, Nakabeppu Y, Yamagata Y.
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 69(Pt 1) Issue: 1 Pages: 45-48

    • DOI

      10.1107/s1744309112048002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] mpact of antibodies against amyloidogenic transthyretin (ATTR) on phenotypes of patients with familial amyloidotic polyneuropathy (FAP) ATTR Valine30Methionine.2013

    • Author(s)
      Obayashi, K., Tasaki, M., Jono, H., Ueda, M., Shinriki, S., Misumi, Y., Yamashita, T., Oshima, T., Nakamura, T., Ikemizu, S., Anan, I., Suhr, O., & Ando, Y.
    • Journal Title

      Clin. Chim. Acta

      Volume: 419 Pages: 127-131

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure and interactions of the human programmed cell death 1 receptor.2013

    • Author(s)
      Cheng, X., Veverka, V., Radhakrishnan, A., Waters, L.C., Muskett, F.W., Morgan, S., Huo, J., Yu, C., Evans, E.J., Leslie, A.J., Griffiths, M., Stubberfield, C., Griffin, R., Henry, A.J., Jansson, A., Ladbury, J.E., Ikemizu, S., Carr, M.D., Davis, S.J.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Pages: 11771-11785

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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