| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Fertilization begins with species-restricted interaction of sperm and the egg-coating envelope that internalizes a three-dimensional meshwork of filaments constituted by glycoproteins (called ZP proteins). Dicalcin, a novel ZP protein-binding protein in Xenopus egg envelope, suppresses the fertilization rate through its binding to gp41, a frog counterpart of mammalian ZP3. We identified the interactive amino-acid regions between dicalcin and gp41 by using a series of truncated mutants. We next clamped the egg envelope at fertilization competent or incompetent status by extrinsic treatment with synthetic peptides corresponding to interactive regions, and found that there is a striking nanoscale-structural difference between two statuses of mature unfertilized eggs.
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