| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We have partially succeeded in constructing the heterologous expression system for both Fum8 and Fum13, which were the key unit of the Fum gene cluster participating to fumonisin B1 biosynthesis. The Fum13p overproduced in Escherichia coli was homogeneously purified, enzymatically characterized and tried to crystallized. New convenient non-RI assay system for the detection of the precursor of FB1 was constructed by combination of the Fum8p-overexpressed yeast microsomes and the purified Fum13p, and substrates synthesized by bacterial SPT. This assay system is useful for the screening of the inhibitor of the fumonisin biosynthesis.
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