| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this research, we performed a detailed analysis of Rim21 which had been identified by us as the sensor protein for plasma membrane lipid asymmetry. As a result, we succeeded in identifying the sensor motif that directly recognizes the state of lipid asymmetry. We also found that the sensor motif interacts with some of negatively charged lipids. From these and other results, we proposed a model for sensing mechanism of altered lipid asymmetry by Rim21.
On the other hand, we also investigated cellular responses to altered lipid asymmetry. We focused on Opt2 which we previously identified as a key molecule in adapataion to altered lipid asymmetry. In consequence, we revealed that Opt2 mediates exposure of phospholipids to the outer leaflet of the plasma membrane.
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