The molecular mechanism for fundamental properties of circadian clock, input, oscillation, and output
| Project/Area Number |
25440041
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
YOSHITANE Hikari 東京大学, 理学(系)研究科(研究院), 助教 (70569920)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | サーカディアンリズム / 体内時計 / ChIP-Seq解析 / RNA-Seq解析 / インタラクトーム解析 / 転写制御 / バイオインフォマティクス / 生物時計 / 時計遺伝子 / CLOCK / BMAL1 / ChIP-Seq / RNA-Seq |
|---|
| Outline of Final Research Achievements |
We previously identified CLOCK-binding sites in mouse liver by ChIP-Seq analysis, and in this study a newly developed bioinformatics method, motif centrality analysis of ChIP-Seq (MOCCS), revealed CACGNG, CACGTT, and CATG[T/C]G as functional E-box sequences targeted by CLOCK. We reported Nrf2 and Nfatc2 genes as rhythmic output genes from the E-boxes. Functional D-box sequences targeted by DBP protein were determined by ChIP-Seq analysis. We also identified CaMKII, UBE3A, and USP7 as a protein kinase for CLOCK phosphorylation, an E3 ligase for BMAL1 ubiquitination, and a de-ubiquitination enzyme for CRY ubiquitination, respectively.
|
Report
(4 results)
Research Products
(57 results)
-
-
[Journal Article] The chondrocyte clock gene Bmal1 controls cartilage homeostasis and integrity2016
Author(s)
Michal Dudek, Nicole Gossan, Nan Yang, Hee-Jeong Im, Jayalath PD Ruckshanthi, Hikari Yoshitane, Xin Li, Ding Jin, Ping Wang, Maya Boudiffa, Ilaria Bellantuono, Yoshitaka Fukada, Ray P. Boot-Handford, and Qing-Jun Meng.
-
Journal Title
The Journal of Clinical Investigation
Volume: 126
Issue: 1
Pages: 365-376
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] CLOCK-controlled polyphonic regulations of circadian rhythms through canonical and non-canonical E-boxes.2014
Author(s)
Hikari Yoshitane, Haruka Ozaki, Hideki Terajima, Ngoc-Hien Du, Yutaka Suzuki, Taihei Fujimori, Naoki Kosaka, Shigeki Shimba, Sumio Sugano, Toshihisa Takagi, Wataru Iwasaki, and Yoshitaka Fukada.
-
Journal Title
Molecular and Cellular Biology
Volume: 34
Issue: 10
Pages: 1776-1787
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] The circadian clock regulates the NRF2/ARE-mediated antioxidant defence pathway in the mouse lung.2014
Author(s)
Pekovic-Vaughan V, Gibbs J, Yoshitane H, Yang N, Pathiranage D, Guo B, Sagami A, Taguchi K, Bechtold D, Loudon A, Yamamoto M, Chan J, van der Horst GTJ, Fukada Y, and Meng Q-J.
-
Journal Title
Genes Dev
Volume: 28
Issue: 6
Pages: 548-560
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] CaMKII is essential for the cellular clock and coupling between morning and evening behavioral rhythms.2014
Author(s)
Kon N, Yoshikawa T, Honma S, Yamagata Y, Yoshitane H, Shimizu K, Sugiyama Y, Hara C, Kameshita I, Honma K, Fukada Y.
-
Journal Title
Genes Dev
Volume: 28
Issue: 10
Pages: 1101-1110
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
