| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We previously identified CLOCK-binding sites in mouse liver by ChIP-Seq analysis, and in this study a newly developed bioinformatics method, motif centrality analysis of ChIP-Seq (MOCCS), revealed CACGNG, CACGTT, and CATG[T/C]G as functional E-box sequences targeted by CLOCK. We reported Nrf2 and Nfatc2 genes as rhythmic output genes from the E-boxes. Functional D-box sequences targeted by DBP protein were determined by ChIP-Seq analysis. We also identified CaMKII, UBE3A, and USP7 as a protein kinase for CLOCK phosphorylation, an E3 ligase for BMAL1 ubiquitination, and a de-ubiquitination enzyme for CRY ubiquitination, respectively.
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