| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The mucin-like membrane protein Msb2, the membrane anchor protein Opy2, and the adaptor protein Ste50 play important roles in activation of the HOG MAPK pathway and the FG MAPK pathway. When a cytoplasmic B site of Opy2 is phosphorylated by casein kinases Yck1/Yck2 under nutrient-rich conditions, it binds Ste50 and only leads to activation of the HOG pathway. Mutants were isolated that have mutations in genes encoding molecules, that negatively regulate phosphorylation of the B site. Extracellular binding between Mab2 and Opy2 was changed by osmostress.
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