Regulation mechanism of MAPK pathway specificity in yeast

• Project/Area Number: 25440042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Yamamoto Katsuyoshi 東京大学, 医科学研究所, 特任助教 (70508366)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞外システイン・リッチ領域の構造 / 浸透圧センサー / シグナル伝達 / MAPキナーゼ / HOG経路 / 酵母 / 出芽酵母

Outline of Final Research Achievements

The mucin-like membrane protein Msb2, the membrane anchor protein Opy2, and the adaptor protein Ste50 play important roles in activation of the HOG MAPK pathway and the FG MAPK pathway. When a cytoplasmic B site of Opy2 is phosphorylated by casein kinases Yck1/Yck2 under nutrient-rich conditions, it binds Ste50 and only leads to activation of the HOG pathway. Mutants were isolated that have mutations in genes encoding molecules, that negatively regulate phosphorylation of the B site. Extracellular binding between Mab2 and Opy2 was changed by osmostress.

Research Products

• [Journal Article] Binding of the extracellular eight-cysteine motif of Opy2 to the putative osmosensor Msb2 is essential for activation of the yeast High-Osmolarity Glycerol pathway (2016)
  • Author(s): Yamamoto K, Tatebayashi K, and Saito H
  • Journal Title: Molecular and Cellular Bioloby Volume: 36 Issue: 3 Pages: 475-487
  • DOI: 10.1128/mcb.00853-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase. (2016)
  • Author(s): Nishimura A, Yamamoto K, Oyama M, Kozuka-Hata H, Saito H, Tatebayashi K.
  • Journal Title: Mol Cell Biol Volume: 36 Issue: 7 Pages: 1109-1123
  • DOI: 10.1128/mcb.01017-15
  • Peer Reviewed
• [Journal Article] Osmosensing and scaffolding functions of the oligomeric four-transmembrane domain osmosensor Sho1. (2015)
  • Author(s): Tatebayashi K, Yamamoto K, Nagoya M, Takayama T, Nishimura A, Sakurai M, Momma T, and Saito H.
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6975-6975
  • DOI: 10.1038/ncomms7975
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms (2014)
  • Author(s): K. Tanaka, K. Tatebayashi, A. Nishimura, K. Yamamoto, H.Y. Yang, and H. Saito
  • Journal Title: Science Signaling Volume: 7 Issue: 314 Pages: 1-10
  • DOI: 10.1126/scisignal.2004780
  • Peer Reviewed
• [Presentation] 浸透圧応答を制御する出芽酵母HOG MAPキナーゼ経路における膜蛋白質Opy2の細胞外cysteine-rich領域の役割 (2014)
  • Author(s): 山本勝良、西村晶子、舘林和夫、斎藤春雄
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-26
• [Presentation] 高浸透圧ストレス応答HOG MAPK経路活性化に必須な膜蛋白質Opy2の細胞外システイン・リッチ領域の解析
  • Author(s): 山本勝良、西村晶子、舘林和夫、斎藤春雄
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区））
• [Presentation] 出芽酵母の高浸透圧センサーHkr1の機能解析
  • Author(s): 西村晶子、山本勝良、舘林和夫、斎藤春雄
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区））
• [Presentation] 四回膜貫通型Sho1高浸透圧共センサーが形成する二次元六角格子状多量体構造とそのシグナル伝達への関与
  • Author(s): 舘林和夫、山本勝良、奈古屋美穂、西村晶子、斎藤春雄
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区））