Research of phagocytosis-mediated regulation of growth rate in Drosophila

• Project/Area Number: 25440044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University (2014-2015); Kanazawa University (2013)
• Principal Investigator: NAGAOSA Kaz 弘前大学, 食料科学研究所, 准教授 (70401891)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 貪食 / 成長調節 / ショウジョウバエ / 転写 / アポトーシス / 自然免疫 / 死細胞 / 成長 / 貪食受容体 / 発生

Outline of Final Research Achievements

Unwanted cells are induced cell death, then phagocytes recognize them using phagocytosis receptor(s) and engulfed. Our previous experiments showed that a Drosophila lacking phagocytosis receptors, Draper and integrin beta-nu, took longer time to develop into adults. This results hypothesized that phagocytosis receptor-mediated phagocytosis controlled ontogenetic growth. To be clear that, phagocytosing phagocyte was subjected to DNA microarray analysis and gel-shift assay. I found; 1) expression level of growth-related gene B was up-regulated, 2) amount of a repressor C which suppresses transcription of the gene B was decreased, and 3) another repressor A which suppresses transcription of the gene C was activated. These findings support the hypothesis.

Research Products

• [Journal Article] Peptidoglycan recognition protein-triggered induction of Escherichia coli gene in Drosophila melanogaster (2015)
  • Author(s): Kong, Q., Nakai, Y., Kuroda, N., Shiratsuchi, A., Nagaosa, K., and Nakanishi, Y.
  • Journal Title: J. Biochem. Volume: 00 Issue: 6 Pages: 00-00
  • DOI: 10.1093/jb/mvv002
  • NAID: 120005680039
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ショウジョウバエを用いた新規ながん予防食品探索法の開発 (2016)
  • Author(s): 永長一茂, 前多隼人
  • Organizer: 日本農芸化学会2016年度札幌大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-28
• [Presentation] ショウジョウバエによるリンゴの癌予防効果の解析 (2015)
  • Author(s): 永長一茂
  • Organizer: 赤い果肉リンゴを核とした地域活性化シンポジウム
  • Place of Presentation: ホテルニューキャッスル（青森県弘前市）
  • Year and Date: 2015-12-22
• [Presentation] 変異細胞の除去を促す食材を見出すための動物実験法の開発 (2015)
  • Author(s): 永長一茂
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-02
• [Presentation] Identification of the ligand-receptor system that governs tumor-suppressive cell competition (2015)
  • Author(s): Kaz Nagaosa
  • Organizer: 1st International Symposium on Cell Competition
  • Place of Presentation: Shiran Kaikan (Kyoto, Japan)
  • Year and Date: 2015-09-10
  • Int'l Joint Research
• [Presentation] ショウジョウバエの個体成長における貪食受容体の役割 (2014)
  • Author(s): 永長 一茂
  • Organizer: 日本生化学会北陸支部第32回大会
  • Place of Presentation: 富山
  • Year and Date: 2014-05-24
  • Invited
• [Presentation] ショウジョウバエにおけるホスファチジルセリンを介したアポトーシス細胞貪食の解析 (2013)
  • Author(s): Tran Thanh Tung, 永長一茂, 藤田融, 喜多朝菜, 森大樹, 岡田亮, 野中さおり, 中西義信
  • Organizer: 86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 貧栄養下でのショウジョウバエの個体成長における貪食受容体の役割
  • Author(s): 永長一茂, 喜多朝菜, 野中さおり, 中西義信
  • Organizer: 日本生化学会北陸支部第31回大会
  • Place of Presentation: 金沢
• [Presentation] ショウジョウバエの個体成長における貪食受容体の役割
  • Author(s): 永長一茂
  • Organizer: 日本生化学会北陸支部第3２回大会
  • Place of Presentation: 富山
  • Invited