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Molecular mechanism underlying cell adhesion to basement membrane laminins

Research Project

Project/Area Number 25440047
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

Yamada Masashi  大阪大学, たんぱく質研究所, 助教 (90304055)

Co-Investigator(Renkei-kenkyūsha) SEKIGUCHI Kiyotoshi  大阪大学, たんぱく質研究所, 教授 (50187845)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞外マトリックス / 基底膜 / ラミニン / インテグリン / テトラスパニン / 上皮極性 / 癌 / 三次元培養 / 細胞遊走
Outline of Final Research Achievements

Analysis of a disease-associated mutation of the laminin-binding integrin α3: A missense mutation that causes substitution of Arg628 with Pro (R628P) in the calf-1 domain of human α3 was shown to be associated with disorders of the lung, kidney, and skin. We found that the R628P mutation leads to aberrations in the posttranslational processing of α3. This mutation abolished the interaction of α3 with the tetraspanin CD151 but not integrin β1.
The role of laminin-binding integrins in epithelial polarity: To elucidate the mechanism underlying epithelial polarity formation, we employed and compared tumor and non-tumor cell lines with distinct abilities to develop epithelial polarity. We found that expression levels of laminin-binding integrins were quite low in some cancer cells compared with non-tumor cells. Furthermore, the exogenous expression of the laminin-binding integrin in these cells promoted the formation of epithelial polarity in 3D culture.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Necdin controls EGFR signaling linked to astrocyte differentiation in primary cortical progenitor cells.2016

    • Author(s)
      Fujimoto, I., Hasegawa, K., Fujiwara, K., Yamada, M., Yoshikawa, K.
    • Journal Title

      Cellular Signalling

      Volume: 28 Issue: 2 Pages: 94-107

    • DOI

      10.1016/j.cellsig.2015.11.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecular basis of laminin-integrin interactions.2015

    • Author(s)
      Yamada, M., Sekiguchi, K.
    • Journal Title

      Curr. Top. Membr.

      Volume: 76 Pages: 197-229

    • DOI

      10.1016/bs.ctm.2015.07.002

    • ISBN
      9780128040157
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular basis of laminin-integrin interactions2015

    • Author(s)
      Masashi Yamada, Kiyotoshi Sekiguchi
    • Journal Title

      Current Topics in Membranes

      Volume: 未定

    • Related Report
      2014 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] Disease-associated single amino acid mutation in the calf-1 domain of integrin α3 leads to defects in its processing and cell surface expression.2013

    • Author(s)
      Yamada, M., and Sekiguchi, K.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 441 Issue: 4 Pages: 988-993

    • DOI

      10.1016/j.bbrc.2013.11.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Substrate-attached materials are enriched with tetraspanins and are analogous to the structures associated with rear-end retraction in migrating cells.2013

    • Author(s)
      Yamada, M., Mugnai, G., Serada, S., Yagi, Y., Naka, T., and Sekiguchi K.
    • Journal Title

      Cell Adh. Migr.

      Volume: 7 Issue: 3 Pages: 304-314

    • DOI

      10.4161/cam.25041

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nephronectin binds to heparan sulfate proteoglycans via its MAM domain2013

    • Author(s)
      佐藤 祐哉
    • Journal Title

      Matrix Biology

      Volume: VOL. 32 Issue: 3-4 Pages: 188-195

    • DOI

      10.1016/j.matbio.2013.01.005

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 「マウス基底膜ボディマップ」データベース2015

    • Author(s)
      二木杉子、筒井仰、眞鍋理一郎、山田雅司、関口清俊、大槻勝紀
    • Organizer
      第56回日本組織細胞化学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 上皮細胞の頂底極性形成におけるラミニン結合性インテグリンの役割2015

    • Author(s)
      山田雅司、永野雄大、富本千晶、宇佐美晶子、佐藤(西内)涼子、関口清俊
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Presentation] Ⅰ型コラーゲン線維上培養ヒト肺ガン細胞株、A549細胞の上皮-間葉転換(EMT)と2次元遊走制御について2015

    • Author(s)
      藤崎ひとみ、二木杉子、山田雅司、関口清俊、服部俊治
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] The involvement of the extracellular matrix protein polydom in the regulation of mouse bone marrow mesenchymal stem cells2015

    • Author(s)
      Masashi Yamada, Hiroshi Shitamichi, Ryoko Sato-Nishiuchi, Kenji Kusumoto, Nanami Morooka, Katsuto Tamai, Sachiko Ezoe, Sugiko Futaki, Kiyotoshi Sekiguchi
    • Organizer
      International Society for Stem Cell Research 2015
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2015-06-24 – 2015-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] The involvement of the extracellular matrix protein polydom in the regulation of mouse bone marrow mesenchymal stem cells.2015

    • Author(s)
      Yamada, M., Shitamichi, H., Sato-Nishiuchi, R., Kusumoto, K., Morooka, N., Tamai, K., Ezoe, S., Futaki, S., Sekiguchi, S.
    • Organizer
      International Society for Stem Cell Research 2015
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒト乳癌MCF-7細胞におけるラミニン結合性インテグリン依存的な上皮極性形成2015

    • Author(s)
      山田雅司、永野雄大、宇佐美晶子、富本千晶、佐藤(西内)涼子、関口清俊
    • Organizer
      第47回日本結合組織学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
  • [Presentation] Ⅰ型コラーゲン線維上培養ヒト肺ガン細胞株、A549細胞の上皮-間葉転換(EMT)と2次元遊走制御について2015

    • Author(s)
      藤崎ひとみ、二木杉子、山田雅司、関口清俊、服部俊治
    • Organizer
      第47回日本結合組織学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 2種のヒト皮膚表皮角化細胞株、HaCat細胞とFEPE1L-8細胞によるI型コラーゲンへの接着感受性比較検討2014

    • Author(s)
      藤崎ひとみ、二木杉子、池島喬、林利彦、山田雅司、関口清俊、服部俊治
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 基底膜ラミニンによるインテグリンおよびテトラスパニンを介した上皮細胞機能制御2014

    • Author(s)
      山田雅司、関口清俊
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ヒトの疾患原因となるインテグリンα3のR628P変異はそのプロッセッシングと細胞表面発現の異常をもたらす

    • Author(s)
      山田雅司、関口清俊
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学・蛋白質研究所・細胞外マトリックス研究室

    • URL

      http://www.protein.osaka-u.ac.jp/chemistry/

    • Related Report
      2014 Research-status Report
  • [Remarks] 大阪大学・蛋白質研究所・細胞外マトリックス研究室

    • URL

      http://www.protein.osaka-u.ac.jp/chemistry/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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