Molecular mechanism underlying cell adhesion to basement membrane laminins
| Project/Area Number |
25440047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Yamada Masashi 大阪大学, たんぱく質研究所, 助教 (90304055)
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| Co-Investigator(Renkei-kenkyūsha) |
SEKIGUCHI Kiyotoshi 大阪大学, たんぱく質研究所, 教授 (50187845)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 細胞外マトリックス / 基底膜 / ラミニン / インテグリン / テトラスパニン / 上皮極性 / 癌 / 三次元培養 / 細胞遊走 |
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| Outline of Final Research Achievements |
Analysis of a disease-associated mutation of the laminin-binding integrin α3: A missense mutation that causes substitution of Arg628 with Pro (R628P) in the calf-1 domain of human α3 was shown to be associated with disorders of the lung, kidney, and skin. We found that the R628P mutation leads to aberrations in the posttranslational processing of α3. This mutation abolished the interaction of α3 with the tetraspanin CD151 but not integrin β1.
The role of laminin-binding integrins in epithelial polarity: To elucidate the mechanism underlying epithelial polarity formation, we employed and compared tumor and non-tumor cell lines with distinct abilities to develop epithelial polarity. We found that expression levels of laminin-binding integrins were quite low in some cancer cells compared with non-tumor cells. Furthermore, the exogenous expression of the laminin-binding integrin in these cells promoted the formation of epithelial polarity in 3D culture.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Substrate-attached materials are enriched with tetraspanins and are analogous to the structures associated with rear-end retraction in migrating cells.2013
Author(s)
Yamada, M., Mugnai, G., Serada, S., Yagi, Y., Naka, T., and Sekiguchi K.
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Journal Title
Cell Adh. Migr.
Volume: 7
Issue: 3
Pages: 304-314
DOI
Related Report
Peer Reviewed
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[Presentation] The involvement of the extracellular matrix protein polydom in the regulation of mouse bone marrow mesenchymal stem cells.2015
Author(s)
Yamada, M., Shitamichi, H., Sato-Nishiuchi, R., Kusumoto, K., Morooka, N., Tamai, K., Ezoe, S., Futaki, S., Sekiguchi, S.
Organizer
International Society for Stem Cell Research 2015
Place of Presentation
Stockholm, Sweden
Year and Date
2015-06-24
Related Report
Int'l Joint Research
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