| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Nematode was used as a model for the elucidation of physiological function of b561 protein. Of the seven b561 homologs of the nematode, we studied three of them, i.e., Cecytb-5, Cecytb-1, Cecytb-2. Cecytb-1 was found to be expressed in pharynx and ovary, and was thought to be related to the neurotransmitter biosynthesis. Cecytb-5 was found to be expressed in the ovary. We previously found that Cecytb-2 is expressed in the intestine and has a function as Dcytb in the nematodes. We constructed the expression system for these proteins using methanol-assimilating yeast and, for the first time, succeeded in the expression and purification of both Cecytb-1 and Cecytb-5 proteins. Both proteins can be prepared as a heme-binding holo-type and showed a rapid reduction by ascorbic acid.
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