Physiological molecular functions of cytochrome b561 in Caenorhabditis elegans
Project/Area Number |
25440048
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
|
Research Institution | Kobe University |
Principal Investigator |
Tsubaki Motonari 神戸大学, 理学(系)研究科(研究院), 教授 (00145046)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 膜タンパク質 / cytochrome b561 / ビタミンC / アスコルビン酸 / 鉄代謝 / 電子伝達 / シトクロムb561 / Caenorhabditis elegans / ヘムタンパク質 / 神経伝達物質 / 線虫 / 資化性酵母 / シトクロム / 異種発現系 |
Outline of Final Research Achievements |
Nematode was used as a model for the elucidation of physiological function of b561 protein. Of the seven b561 homologs of the nematode, we studied three of them, i.e., Cecytb-5, Cecytb-1, Cecytb-2. Cecytb-1 was found to be expressed in pharynx and ovary, and was thought to be related to the neurotransmitter biosynthesis. Cecytb-5 was found to be expressed in the ovary. We previously found that Cecytb-2 is expressed in the intestine and has a function as Dcytb in the nematodes. We constructed the expression system for these proteins using methanol-assimilating yeast and, for the first time, succeeded in the expression and purification of both Cecytb-1 and Cecytb-5 proteins. Both proteins can be prepared as a heme-binding holo-type and showed a rapid reduction by ascorbic acid.
|
Report
(4 results)
Research Products
(51 results)