| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Biochemical characterization of functional enzymes involved in L-hydroxyproline metabolism from bacteria were carried out. D-Hydroxyproline dehydrogenase consisted of α-,β- and γ-subunits, in which the β-subunit played a role as a catalytic subunit, and the remaining them functioned to maintain the structural folding and/or to improve the catalysis. A lysine residue at the position of 164 formed a Schiff base intermediate under NaBH4. Several bacteria could metabolize not only 4-hydroxyproline but also 3-hydroxyproline, and trans-3-hydroxy-L-proline dehydratase and Δ1-pyrroline 2-carboxylate reductase involved in the 3-hydroxyptoline metabolism were identified enzymatically and genetically.
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