| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
With improving the conditions of gene expression, host cell culture, and enzyme purification, we have succeeded in getting a highly resolved stereostructure of cytochrome bd-type menaquinol oxidase in respiratory chain of Gram-positive thermoplilic bacterium Geobacillus thermodenitirificans K1041 strain, based on a collaboration with X-ray crystallography technique by foreign researchers. This is the first atomic structure of cytochrome bd-type oxidase and does not resemble to the heme-copper oxidases or any other known proteins at all.
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