Roles of ARFs in superoxide production and chemotaxis of neutrophil

• Project/Area Number: 25440051
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hokkaido University (2014-2015); University of Occupational and Environmental Health, Japan (2013)
• Principal Investigator: MAZAKI Yuichi 北海道大学, 医学(系)研究科(研究院), 講師 (60311304)
• Co-Investigator(Renkei-kenkyūsha): SABE Hisataka 北海道大学, 医学研究科, 教授 (40187282)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 好中球 / 遊走 / 活性酸素

Outline of Final Research Achievements

Neutrophils are important in innate immunity and in the initiating an acute response to infection. During such a response, neutrophils migrate towards invaders and produce antimicrobial agents, including many reactive oxygen species. Previously, we reported that ARF regulatory factors, which are membrane trafficking protein, are involved in superoxide production and chemotaxis of neutrophils. In this study, we examined roles of ARFs in superoxide production and chemotaxis of neutrophil. Our results suggest that ARF1 is involved in subcellular localization of Rac1 during chemotaxis.

Research Products

• [Journal Article] Endothelin-1 suppresses insulin-stimulated Akt phosphorylation and glucose uptake via G protein- coupled receptor kinase 2 in skeletal muscle cells. (2016)
  • Author(s): Horinouchi, T., Hoshi, A., Harada, T., Higa, T., Karki, S., Terada, K., Higashi, T., Mai, Y., Nepal, P., Mazaki, Y., Miwa. S.
  • Journal Title: Br. J. Pharmacol Volume: 173 Issue: 5 Pages: 1018-1032
  • DOI: 10.1002/asia.201501347
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overexpression of Peroxiredoxin 4 Induces Adaptive Intestinal Lipid Metabolism Associated with Suppressive Nonalcoholic Steatohepatitis in a Dietary Mouse Model. (2016)
  • Author(s): Aya Nawata, Hirotsugu Noguchi, Yuichi Mazaki, Toshihiro Kurahashi, Atsunori Nabeshima, Ke-Yong Wang, Satoshi Kimura, Hidetaka Uramoto, Kimitoshi Kohno, Hatsumi Taniguchi, Yoshiya Tanaka, Junichi Fujii, Yasuyuki Sasaguri Toshiyuki Nakayama, and Sohsuke Yamada.
  • Journal Title: PLoS One Volume: e0152549 Issue: 4 Pages: e0152549-e0152549
  • DOI: 10.1371/journal.pone.0152549
  • Peer Reviewed / Open Access
• [Journal Article] A standardized method for preparation of gas phase extract of cigarette smoke. (2016)
  • Author(s): Higashi, T., Mai, Y., Mazaki, Y., Horinouchi, T., Miwa, S.
  • Journal Title: Biol. Pharm. Bull. Volume: 39
  • NAID: 130005155132
  • Acknowledgement Compliant
• [Journal Article] Carbonyl compounds in the gas phase of cigarette mainstream smoke and their pharmacological properties. (2016)
  • Author(s): Horinouchi, T., Higashi, T., Mazaki, Y., Miwa, S.
  • Journal Title: Biol. Pharm. Bull. Volume: 39
  • NAID: 130005155133
  • Acknowledgement Compliant
• [Presentation] タバコ煙ガス相水抽出液の簡便な標準的作製法の開発 (2016)
  • Author(s): 真崎雄一
  • Organizer: 日本薬理学会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-03-10
• [Presentation] 好中球のケモタキシスにおいてGBF1はARF1を介してRac1の局在に関与する (2014)
  • Author(s): 真崎雄一
  • Organizer: 日本エンドトキシン・自然免疫研究会
  • Place of Presentation: 順天堂大学本郷キャンパス（東京都）
  • Year and Date: 2014-12-06
• [Presentation] HL-60細胞のケモタキシスにおいてArf1はRac1の細胞内局在に関与する (2014)
  • Author(s): 真崎雄一
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂（奈良市）
  • Year and Date: 2014-06-13