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Proposal of hybrid-type chaperonin reaction mechanism in response to the intracellular environment

Research Project

Project/Area Number 25440055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionKanagawa Institute of Technology

Principal Investigator

KOIKE AYUMI  神奈川工科大学, 応用バイオ科学部, 教授 (20454176)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsタンパク質フォールディング / シャペロニン / GroEL / フォールディング / タンパク質
Outline of Final Research Achievements

GroEL is able to use both the “bullet cycle” and the “football cycle”, the selection will depend on the situation in the cell. We determined the crystal structure of the “football” GroEL:GroES2 complex. The overall structure of the “football” complex resembled the GroES-bound GroEL-ring of the asymmetric 1:1 GroEL:GroES complex (the “bullet” complex). However, the two GroES-bound GroEL-rings form a modified interface by a rotation about the 7-fold axis. As a result, the inter-ring contacts between the two GroEL rings in the football complex differed from those in the bullet complex. The altered inter-GroEL ring contacts in the football structure lead GroEL tetradecamer to dissociated in the presence of ATP and GroES in a native gel.
Furthermore, we constructed coexpression system of distinct fluorescence molecules-labeled GroES in E. coli for the purpose of detection of the football-like reaction intermediates by FCCS.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (26 results)

All 2016 2015 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Int'l Joint Research: 4 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Asp52 in combination with Asp398 plays a critical role in ATP hydrolysis of chaperonin GroEL.2014

    • Author(s)
      Koike-Takeshita A, Mitsuoka K, Taguchi H
    • Journal Title

      J. Biol. Chem.

      Volume: 289(43) Issue: 43 Pages: 30005-11

    • DOI

      10.1074/jbc.m114.593822

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a symmetric football-shaped GroEL:GroES2-ATP14 complex determined at 3.8Å reveals rearrangement between two GroEL rings.2014

    • Author(s)
      Koike-Takeshita A, Arakawa T, Taguchi H, Shimamura T.
    • Journal Title

      J Mol Biol.

      Volume: 426 Issue: 21 Pages: 3634-41

    • DOI

      10.1016/j.jmb.2014.08.017

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Monitoring Amyloid Sup35NM Growth with Label-Free Electrical Detection Using Field Effect Transistor Biosensor2014

    • Author(s)
      S. Hideshima, S. Wustoni, S. Kuroiwa, T. Nakanishi, A. K-Takeike, T. Osaka
    • Journal Title

      ChemElectroChem

      Volume: 1 Issue: 1 Pages: 51-54

    • DOI

      10.1002/celc.201300151

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Crystal structure of a symmetric foot-ball-shaped GroEL:GroES2-ATP14 complex reveals rearrangement between two GroEL rings2015

    • Author(s)
      Ayumi Koike-Takeshita, Takatoshi Arakawa, Hideki Taguchi, Tatsuro Shimamura
    • Organizer
      THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ合衆国ハワイ州ホノルル)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of GroEL-GFP protein which holds the chaperonin function: A tool to study elucidation of GroEL molecular mechanism in vivo2015

    • Author(s)
      Yuta Nishimura, Tatsuya Niwa, Hideki Taguchi, Ayumi Koike-Takeshita
    • Organizer
      THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ合衆国ハワイ州ホノルル)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光相関分光法を用いた大腸菌生細胞内でのシャペロニン動態解析2015

    • Author(s)
      西村 友汰、星 健介、丹羽 達也、田口 英樹、小池 あゆみ
    • Organizer
      Biochemistry and Molecular Biology 2015
    • Place of Presentation
      ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Crystal structure of a symmetric foot-ball-shaped GroEL:GroES2-ATP14 complex reveals rearrangement between two GroEL rings2015

    • Author(s)
      Ayumi Koike-Takeshita, Takatoshi Arakawa, Hideki Taguchi, Tatsuro Shimamura
    • Organizer
      Protein Island Matsuyama (PIM) International Symposium 2015
    • Place of Presentation
      松山市男女共同参画推進センター(愛媛県松山市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of GFP fusion GroEL which holds the chaperonin function: a tool to study elucidation of GroEL molecular mechanism in vivo2015

    • Author(s)
      Yuta Nishimura, Hideki Taguchi, Ayumi Koike-Takeshita
    • Organizer
      Protein Island Matsuyama (PIM) International Symposium 2015
    • Place of Presentation
      松山市男女共同参画推進センター(愛媛県松山市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フットボール型複合体 GroEL:GroES2-ATP14の結晶構造解析2015

    • Author(s)
      小池 あゆみ、荒川 孝俊、田口 英樹、島村 達郎
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] シャペロニンGroEL内に形成された金属ナノ粒子の規則配列2014

    • Author(s)
      星野文彦、西田愛、依田ひろみ、小池(竹下)あゆみ
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞内GroELの分子機構解明のためのシャペロニン機能を保持したGFP融合GroELの構築2014

    • Author(s)
      西村友汰、土生勇樹、丹羽達也、田口英樹、小池あゆみ
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Approach fpr an alignment of chaperonin GroEL complexes encapsulating metal nano particles2014

    • Author(s)
      H. Yoda, O. Yamamoto, A. Koike - Takeshita
    • Organizer
      EMNT2014 10th International Simposium on Electrochemical Micro & Nanosystem Technologies
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2014-11-06
    • Related Report
      2014 Research-status Report
  • [Presentation] ファージΦTMAの持つGroES様タンパク質の機能解析2014

    • Author(s)
      近藤恒平、玉腰雅忠、小池あゆみ
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15
    • Related Report
      2014 Research-status Report
  • [Presentation] 種の金属ナノ粒子を内包したシャペロニンGroEL/GroES複合体の形成2014

    • Author(s)
      依田ひろみ,小池あゆみ
    • Organizer
      2014年電気化学会秋季大会
    • Place of Presentation
      北海道大学高等教育推進機構
    • Year and Date
      2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Functional analysis of ΦTMA-encoded GroES like protein2014

    • Author(s)
      K. Kondo, M. Tamakoshi, A. koike-Takeshita
    • Organizer
      第4回モデル生物丸ごと一匹学会
    • Place of Presentation
      大阪大学会館
    • Year and Date
      2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 2種類の金属ナノ粒子を内包する水溶性ナノカプセルとしてのシャペロニンGroEL複合体の利用の検討2014

    • Author(s)
      依田ひろみ,小池あゆみ
    • Organizer
      第66回日本生物工学大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞内GroEL観察のためのシャペロニン活性を保持したGFP融合GroELの構築2014

    • Author(s)
      西村友汰、土生勇樹、佐伯亨、田村祥平、田口英樹、小池あゆみ
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      ワークピア横浜
    • Year and Date
      2014-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 金属ナノ粒子を内包したシャペロニンGroEL/GroES複合体の形成と評価2014

    • Author(s)
      依田ひろみ,山本修,小池あゆみ
    • Organizer
      電気化学会第81回大会
    • Place of Presentation
      関西大学千里キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] シャペロニンGroEL/GroES の細胞内動態観察2013

    • Author(s)
      小池 あゆみ,佐伯 亨,二ノ井 友里,田村 祥平,田口 英樹
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Research-status Report
  • [Presentation] 新生タンパク質- シャペロン間相互作用を大腸菌内で観察する試み2013

    • Author(s)
      中澤 耕己,丹羽 達也,伊藤 耕一,多田隈 尚史,小池 あゆみ,田口 英樹
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Alignment of GroEL complexes which have nano-sized spaces2013

    • Author(s)
      依田ひろみ,山本修,小池あゆみ
    • Organizer
      第65回日本生物工学会大会
    • Place of Presentation
      広島国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] Effect of Amyloid Conformation On the Response of Field Effect Transistor Biosensor to Sup35NM Protein2013

    • Author(s)
      Sho Hideshima, Shofarul Wustoni, Shigeki Kuroiwa, Takuya Nakanishi, Ayumi Koike-Takeshita, and Tetsuya Osaka
    • Organizer
      ECS Meeting 224th
    • Place of Presentation
      San Francisco
    • Related Report
      2013 Research-status Report
  • [Remarks] 神奈川工科大学応用バイオ科学部応用バイオ科学科小池研究室HP

    • URL

      http://www.kait.jp/ug_gr/undergrad/bio/bioscience/academic/koike.html

    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 変異型シャペロニン複合体を利用した細胞内への局所的薬物送達システム用ナノカプセル2016

    • Inventor(s)
      小池あゆみ、依田ひろみ、高村岳樹
    • Industrial Property Rights Holder
      小池あゆみ、依田ひろみ、高村岳樹
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-05-11
    • Related Report
      2015 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 変異型シャペロニン複合体及び薬物送達システム用ナノカプセル2015

    • Inventor(s)
      小池あゆみ、依田ひろみ、高村岳樹
    • Industrial Property Rights Holder
      小池あゆみ、依田ひろみ、高村岳樹
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-100586
    • Filing Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] ナノ粒子ーGroEL蛋白質複合体及びその製造方法2014

    • Inventor(s)
      星野文彦、今枝孝夫、西田愛、小池あゆみ
    • Industrial Property Rights Holder
      株式会社豊田中央研究所、トヨタ自動車株式会社、学校法人幾徳学園
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-082093
    • Filing Date
      2014-04-11
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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