Regulation of heme oxygenase activity by posttranslational modification
Project/Area Number |
25440056
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Kurume University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MATSUI Takanori 久留米大学, 医学部, 講師 (10425233)
TAIRA Junichi 九州工業大学, 情報工学部, 助教 (20549612)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ヘムオキシゲナーゼ / 翻訳後修飾 / ユビキチン / ユビキチン分解 |
Outline of Final Research Achievements |
We investigated the cellular mechanism underlying the degradation of heme oxygenase-1 (HO-1). 1) The turnover of HO-1 induced in HEK293 was significantly attenuated by proteasome inhibitors, suggesting the involvement of a proteasome-mediated pathway. 2) High molecular weight ubiquitin conjugates were co-immunoprecipitated with HO-1 from HEK293 after proteasome inhibition. 3) HO-1 ubiquitination in HEK293 cells was enhanced by zinc chloride, but suppressed with a zinc chelator, suggesting the involvement of a RING-E3 ligase in this process. 4) We identified TRC8 as the E3 ligase responsible for HO ubiquitination and degradation using mass spectrometry, yeast two-hybrid assay and cDNA array.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] DNA Aptamer Raised Against Advanced Glycation End Products (AGEs) Improves Glycemic Control and Decreases Adipocyte Size in Fructose-Fed Rats by Suppressing AGE-RAGE Axis.2015
Author(s)
Ojima, A., Matsui, T., Nakamura, N., Higashimoto, Y., Ueda, S., Fukami, K., Okuda, S., Yamagishi, S.
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Journal Title
Horm. Metab. Res.
Volume: 47
Pages: 253-258
DOI
Related Report
Peer Reviewed
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[Journal Article] Pigment epithelium-derived factor inhibits caveolin-induced interleukin‑8 gene expression and proliferation of human prostate cancer cells.2015
Author(s)
Matsui, T., Ojima, A., Higashimoto, Y., Taira, J., Fukami, K., Yamagishi, S.
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Journal Title
Oncol. Lett.
Volume: 10
Pages: 2644-2648
DOI
Related Report
Peer Reviewed
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[Journal Article] Improvement of heme oxygenase-1-based heme sensor for quantifying free heme in biological samples.2015
Author(s)
Taira, J., Nakashima, Y., Yoshihara, S., Koga, S., Sueda, S., Komatsu, H., Higashimoto, Y., Takahashi, T., Tanioka, N., Shimizu, H., Morimatsu, H., Sakamoto, H.
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Journal Title
Anal. Biochem.
Volume: 489
Pages: 50-52
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of a monoclonal antibody-based ELISA system for glyceraldehyde-derived advanced glycation end products.2015
Author(s)
Matsui, T., Joo, H.D., Lee, J.M., Ju, S.M., Tao, W.H., Higashimoto, Y., Fukami, K., Yamagishi, S.I.
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Journal Title
Immunol. lett.
Volume: 161
Pages: 141-146
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA aptamer raised against advanced glycation end products (AGEs) prevents abnormalities in electroretinogram of experimental diabetic retinopathy.2015
Author(s)
Maeda, S., Matsui, T., Ojima, A., Suematsu, M., Kaseda, K., Higashimoto, Y., Yamakawa, R., Yamagishi, S.
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Journal Title
Ophthalmic Res.
Volume: 54
Pages: 175-180
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA aptamer raised against advanced glycation end products inhibits melanoma growth in nude mice.2014
Author(s)
Ojima, A., Matsui, T., Maeda, S., Takeuchi, M., Inoue, H., Higashimoto, Y., and Yamagishi, S.I.
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Journal Title
Lab. Invest.
Volume: 94
Pages: 422-429
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural basis for the electron transfer from an open form of NADPH-cytochrome P450 oxidoreductase to heme oxygenase.2014
Author(s)
Sugishima, M., Sato, H., Higashimoto, Y., Harada, J., Wada, K., Fukuyama, K. and Noguchi, M.:
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Journal Title
Proc.Natl.Acad.Sci.USA.
Volume: 111
Pages: 2524-2529
DOI
Related Report
Peer Reviewed
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[Journal Article] EprS, an autotransporter protein of Pseudomonas aeruginosa, possessing serine protease activity induces inflammatory responses through protease-activated receptors.2013
Author(s)
Kida, Y., Taira, J., Yamamoto, T., Higashimoto, Y. and Kuwano, K.
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Journal Title
Cell Microbiol.
Volume: 15
Pages: 1168-1181
DOI
Related Report
Peer Reviewed
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[Journal Article] DNA aptamer raised against AGEs blocks the progression of experimental diabetic nephropathy.2013
Author(s)
Kaida, Y., Fukami, K., Matsui, T., Higashimoto, Y., Nishino, Y., Obara, N., Nakayama, Y., Ando, R., Toyonaga, M., Ueda, S., Takeuchi, M., Inoue, H., Okuda, S. and Yamagishi, S.I.
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Journal Title
Diabetes
Volume: 62
Pages: 3241-3250
DOI
Related Report
Peer Reviewed
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[Journal Article] Blockade by phosphorothioate aptamers of advanced glycation end products-induced damage in cultured pericytes and endothelial cells.2013
Author(s)
Higashimoto, Y., Matsui, T., Nishino, Y., Taira, J., Inoue, H., Takeuchi, M. and Yamagishi, S.I.
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Journal Title
Microvasc. Res.
Volume: 94
Pages: 64-70
DOI
Related Report
Peer Reviewed
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