| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Calpain is an intracellular calcium-requiring cysteine protease, functioning to modulate substrate structure and activity by limited proteolysis. The human genome contains 15 different calpain genes. Among them, CAPN7 (previously called PalBH or calpain-7) is most evolutionarily conserved. In the organisms such as yeast and fungi, CAPN7 homologue functions as an activator for a critical transcription factor in adaptation to an increase of environmental pH. To extend our knowledge about this diversely conserved calpain species, this study aimed to examine the role of CAPN7 in higher vertebrates using genetically modified mouse and cultured cells.
In mouse, homozygous knock-out of the Capn7 gene in C57/B6J background results in neonatal lethality. The primary cause of this phenotype still remains unclear; however, it was demonstrated that CAPN7 protease activity is required for the rescue of this phenotype. Potential function of Capn7 by its structure was also suggested.
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