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Development of nanoheater to control temperature of single protein molecules

Research Project

Project/Area Number 25440062
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

Yuichi Inoue  東北大学, 多元物質科学研究所, 助教 (50323499)

Co-Investigator(Renkei-kenkyūsha) ISHIJIMA Akihiko  東北大学, 多元物質科学研究所, 教授 (80301216)
FUKUOKA Hajime  東北大学, 多元物質科学研究所, 助教 (50447190)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords分子モーター / ナノバイオ / 生物物理
Outline of Final Research Achievements

Single carbon nanotubes (CNTs) were developed as a new tool for regulating local temperature and activity of single proteins. Sliding of single actin filaments along single myosin-coated CNTs was observed under fluorescence microscopy. Then one end of the CNT was irradiated by the red laser to produce local temperature jump that induce activation of the motor speed. The speed change was comparable to the temperature jump of 10 K in average. From the measured distribution of temperature along single CNT, calculation of heat transfer with the finite element method suggested the temperature profile around the CNT and high thermal conductivity of ~1540 W/(m.K) in solution. Laser irradiation CNTs could be also used to regulate local change in concentration of reactive oxygen species that induce various diseases including cancer. Therefore, the flexible control of protein function and local targeting on single CNT, will be useful in future applications of CNTs in biophysics and biomedicine.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (32 results)

All 2016 2015 2014 2013 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Acknowledgement Compliant: 7 results,  Open Access: 2 results) Presentation (16 results) (of which Invited: 2 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Local heating of molecular motors using single carbon nanotubes2015

    • Author(s)
      Yuichi Inoue, and Akihiko Ishijima
    • Journal Title

      Biophysical Reviews

      Volume: 8 Issue: 1 Pages: 25-32

    • DOI

      10.1007/s12551-015-0185-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Torque-induced precession of bacterial flagella2015

    • Author(s)
      Yuji Shimogonya, Yoichiro Sawano, Hiromichi Wakebe, Yuichi Inoue, Akihiko Ishijima & Takuji Ishikawa
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 18488-18488

    • DOI

      10.1038/srep18488

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Dynein arms are strain-dependent direction-switching force generators.2015

    • Author(s)
      Shingyoji, C., Nakano, I., Inoue, Y. and Higuchi, H.
    • Journal Title

      Cytoskeleton

      Volume: 72 Issue: 8 Pages: 388-401

    • DOI

      10.1002/cm.21232

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Giant acceleration of diffusion observed in a single-molecule experiment on F1-ATPase2015

    • Author(s)
      R. Hayashi, K. Sasaki, S. Nakamura, S. Kudo, Y. Inoue, H. Noji and K. Hayashi
    • Journal Title

      Phys. Rev. Lett.

      Volume: 114 Issue: 24 Pages: 248101-248101

    • DOI

      10.1103/physrevlett.114.248101

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single Carbon Nanotube-Based Reversible Regulation of Biological Motor Activity2015

    • Author(s)
      Yuichi Inoue, Mitsunori Nagata, Hiroshi Matsutaka, Takeru Okada, Masaaki K. Sato, and Akihiko Ishijima
    • Journal Title

      ACS Nano

      Volume: 9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dynamic transformations of self-assembled polymeric microspheres induced by AC voltage and shear flow2015

    • Author(s)
      :Morimoto, N.,Wazawa, T., Inoue, Y., Suzukia, M.
    • Journal Title

      RSC Advances

      Volume: 5 Issue: 19 Pages: 14851-14857

    • DOI

      10.1039/c4ra17056c

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temperature changes in brown adipocytes detected with a bimaterial microcantilever2014

    • Author(s)
      Masaaki K. Sato, Masaya Toda, Naoki Inomata, Hisataka Maruyama, Yuko Okamatsu-Ogura, Fumihito Arai, Takahito Ono, Akihiko Ishijima, and Yuichi Inoue
    • Journal Title

      Biophysical Journal

      Volume: 106 Issue: 11 Pages: 2458-2464

    • DOI

      10.1016/j.bpj.2014.04.044

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis.2014

    • Author(s)
      1.Hajime Fukuoka, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, and Akihiko Ishijima.
    • Journal Title

      Science Signaling

      Volume: 319 Issue: 319

    • DOI

      10.1126/scisignal.2004963

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Single-Cell E. coli Response to an Instantaneously Applied Chemotactic Signal2014

    • Author(s)
      Takashi Sagawa, Yu Kikuchi, Yuichi Inoue, Hiroto Takahashi, Takahiro Muraoka, Kazushi Kinbara, Akihiko Ishijima and Hajime Fukuoka
    • Journal Title

      Biophysical Jouranl

      Volume: 107 Issue: 3 Pages: 730-739

    • DOI

      10.1016/j.bpj.2014.06.017

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Micrometer-Size Vesicle Formation Triggered by UV Light2014

    • Author(s)
      Tatsuya Shima, Takahiro Muraoka, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Akihiko Ishijima, Yuki Omata, Takashi Yamashita and Kazushi Kinbara
    • Journal Title

      Langmuir

      Volume: 30 Issue: 25 Pages: 7289-7295

    • DOI

      10.1021/la5008022

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Self-assembled microspheres driven by dipole-dipole interactions: UCST type transition in water2014

    • Author(s)
      Nobuyuki Morimoto, Kanna Muramatsu, Tetsuichi Wazawa, Yuichi Inoue and Makoto Suzuki
    • Journal Title

      Macromolecular Rapid Communications

      Volume: 35 Issue: 1 Pages: 103-108

    • DOI

      10.1002/marc.201300798

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temperature Dependences of Torque Generation and Membrane Voltage in the Bacterial Flagellar Motor.2013

    • Author(s)
      Yuichi Inoue, Matthew A.B. Baker, Hajime Fukuoka, Hiroto Takahashi, Richard M. Berry, Akihiko Ishijima.
    • Journal Title

      Biophysical Journal

      Volume: 105 Issue: 12 Pages: 2801-2810

    • DOI

      10.1016/j.bpj.2013.09.061

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] バクテリアべん毛モーターの2軌道回転運動計測2016

    • Author(s)
      澤野 耀一郎、井上裕一、石島秋彦
    • Organizer
      2016年 生体運動合同班会議
    • Place of Presentation
      キャンパスプラザ京都(京都市)
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新しい熱プローブを用いた細胞発熱計測および分子モーター活性制御2015

    • Author(s)
      井上裕一
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] バクテリアべん毛モーターにおける2軌道回転2015

    • Author(s)
      澤野 耀一郎、井上裕一、石島秋彦
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] カーボンナノチューブを用いたべん毛モーターの抑制的制御2015

    • Author(s)
      井上裕一、澤野耀一郎、福岡創、高橋泰人、石島秋彦
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 局所レーザー照射によるべん毛モーターの活性制御2015

    • Author(s)
      井上 裕一
    • Organizer
      第20回べん毛研究交流会
    • Place of Presentation
      三重県志摩市
    • Year and Date
      2015-03-01 – 2015-03-03
    • Related Report
      2014 Research-status Report
  • [Presentation] 局所レーザー照射による生体分子モーターの活性制御2015

    • Author(s)
      井上 裕一, 永田 光範, 石島 秋彦
    • Organizer
      生体運動合同班会議2015
    • Place of Presentation
      東京
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Research-status Report
  • [Presentation] カーボンナノチューブ1本を用いて生体分子モーター活性を操る2014

    • Author(s)
      井上裕一
    • Organizer
      第14回 東北大学多元物質科学研究所 研究発表会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 高負荷におけるバクテリアべん毛モーターの回転ステップ解析2014

    • Author(s)
      井上 裕一, 福岡 創, 高橋 泰人, 石島 秋彦
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Regulation of the rotational switching of bacterial flagellar motor by binding of an intracellular signaling protein CheY2014

    • Author(s)
      Hajime Fukuoka, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, Akihiko Ishijima
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ウニ精子のカルシウム依存性鞭毛運動制御における脂質ラフト形成2014

    • Author(s)
      中野 泉, 井上 裕一, 金澤 尊, 北島 健, 真行寺 千佳子
    • Organizer
      日本動物学会 第85回仙台大会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 回転ステップ運動の高時間分解能検出に向けた高 S/N 計測系の開発2014

    • Author(s)
      井上 裕一
    • Organizer
      生物物理学会・東北支部会
    • Place of Presentation
      盛岡市
    • Year and Date
      2014-08-25
    • Related Report
      2014 Research-status Report
  • [Presentation] タンパク質単分子の構造変化を計測する暗視野顕微鏡の開発2014

    • Author(s)
      鎌形清人, 間野絵梨子, 七谷圭, 阿蘇裕樹, 井上裕一, 小井川浩之, 高橋聡
    • Organizer
      第14回日本蛋白科学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 楕円体プローブを用いたバクテリアべん毛モーターの角度ステップ解析2014

    • Author(s)
      井上裕一, 分部寛道, 佐川貴志, 福岡創, 石島秋彦
    • Organizer
      2014年 生体運動研究合同班会議
    • Place of Presentation
      千葉市
    • Related Report
      2013 Research-status Report
  • [Presentation] カーボンナノチューブ上におけるアクトミオシン運動と温度分布解析2013

    • Author(s)
      井上裕一
    • Organizer
      新学術領域研究「水を主役としたATPエネルギー変換」終了公開シンポジウム
    • Place of Presentation
      東京都千代田区
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of angular steps of bacterial flagellar motors using an elliptic probe2013

    • Author(s)
      井上 裕一, 分部 寛道 , 佐川 貴志, 福岡 創 , 石島 秋彦
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都市
    • Related Report
      2013 Research-status Report
  • [Presentation] 高負荷時におけるバクテリアべん毛モーターの角度ステップ解析2013

    • Author(s)
      井上裕一
    • Organizer
      生物物理学会東北支部会2013
    • Place of Presentation
      仙台市
    • Related Report
      2013 Research-status Report
  • [Book] 化学, 生体分子モーター機能を操る –生体分子活性のリアルタイム可逆制御を目指して2015

    • Author(s)
      井上裕一、石島秋彦
    • Total Pages
      2
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report
  • [Book] 1分子生物学2014

    • Author(s)
      原田慶恵・石渡信一 編
    • Total Pages
      292
    • Publisher
      化学同人
    • Related Report
      2014 Research-status Report
  • [Remarks] 石島研ホームページ

    • URL

      http://www.tagen.tohoku.ac.jp/labo/ishijima/Index-J-tate.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 石島研究室ホームページ

    • URL

      http://www.tagen.tohoku.ac.jp/labo/ishijima/Index-J-tate.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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