Development of nanoheater to control temperature of single protein molecules
Project/Area Number |
25440062
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Tohoku University |
Principal Investigator |
Yuichi Inoue 東北大学, 多元物質科学研究所, 助教 (50323499)
|
Co-Investigator(Renkei-kenkyūsha) |
ISHIJIMA Akihiko 東北大学, 多元物質科学研究所, 教授 (80301216)
FUKUOKA Hajime 東北大学, 多元物質科学研究所, 助教 (50447190)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 分子モーター / ナノバイオ / 生物物理 |
Outline of Final Research Achievements |
Single carbon nanotubes (CNTs) were developed as a new tool for regulating local temperature and activity of single proteins. Sliding of single actin filaments along single myosin-coated CNTs was observed under fluorescence microscopy. Then one end of the CNT was irradiated by the red laser to produce local temperature jump that induce activation of the motor speed. The speed change was comparable to the temperature jump of 10 K in average. From the measured distribution of temperature along single CNT, calculation of heat transfer with the finite element method suggested the temperature profile around the CNT and high thermal conductivity of ~1540 W/(m.K) in solution. Laser irradiation CNTs could be also used to regulate local change in concentration of reactive oxygen species that induce various diseases including cancer. Therefore, the flexible control of protein function and local targeting on single CNT, will be useful in future applications of CNTs in biophysics and biomedicine.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Micrometer-Size Vesicle Formation Triggered by UV Light2014
Author(s)
Tatsuya Shima, Takahiro Muraoka, Tsutomu Hamada, Masamune Morita, Masahiro Takagi, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Akihiko Ishijima, Yuki Omata, Takashi Yamashita and Kazushi Kinbara
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Journal Title
Langmuir
Volume: 30
Issue: 25
Pages: 7289-7295
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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