Study of functional structure of protein and biomembrane by the developement of resonant X-ray solution scattering in wide-energy range

• Project/Area Number: 25440063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Gunma University
• Principal Investigator: Hirai Mitsuhiro 群馬大学, 大学院理工学府, 教授 (00189820)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: X線散乱 / タンパク質 / 生体膜 / 共鳴散乱 / X線 / 脂質膜 / 折畳み / 共鳴X線散乱 / 吸収端 / 溶液 / 複合体 / 広角散乱

Outline of Final Research Achievements

The use of anomalous dispersion of X-ray (resonant X-ray scattering) is expected to serve us a new insight on functional structures of proteins and membrane in solutions, especially of metalloproteins proteins containing metal atoms or clusters that are involved in a wide range of important biological processes. On the other hand, the recent development of the wide-angle X-ray scattering technique of proteins and membrane using third-generation synchrotron sources shows us that the X-ray solution scattering enables to observe a whole hierarchical structure ranging from ~0.2 nm to ~250 nm, namely, from the secondary structure to the quaternary one of proteins or from the alkylchain packing to the whole structure of lipid aggregate.
The aim of this study was the development of the new method for studying the internal structure of biological material in-situ by the combination of resonant and wide-angle scattering of X-ray.

Research Products

• [Journal Article] Effect of Protein-Encapsulation on Thermal Structural Stability of Liposome Composed of Glycosphingolipid/Cholesterol/Phospholipid (2015)
  • Author(s): Mitsuhiro Hirai, Shoki Sato, Ryota Kimura, Yoshihiko Hagiwara, Rika Kawai-Hirai, Noboru Ohta, Noriyuki Igarashi, and Nobutaka Shimizu
  • Journal Title: J. Phys. Chem. B Volume: 119 Issue: 8 Pages: 3398-3406
  • DOI: 10.1021/jp511534u
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Normalization of grazing-incidence small angle scattering of phospholipid alloy systems (2014)
  • Author(s): H.Okuda, T.Yamamoto,K.Takshita, M.Hirai, K.Senoo, H.Ogawa and Y.Kitajima
  • Journal Title: Jpn. J. Appl. Phys Volume: 53 Issue: 5S1 Pages: 1-5
  • DOI: 10.7567/jjap.53.05fh02
  • Peer Reviewed
• [Journal Article] Conformational characterization of a protein complex involving intrinsically disordered protein by small-angle neutron scattering using the inverse contrast matching method: a case study of interaction between α-synuclein and PbaB tetramer as a model chaperone (2014)
  • Author(s): Masaaki Sugiyama
  • Journal Title: Journal Applied Crystallography Volume: 47 Issue: 1 Pages: 430-435
  • DOI: 10.1107/s1600576713033475
  • Peer Reviewed
• [Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling (2013)
  • Author(s): Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N
  • Journal Title: Journal of Synchrotron Radiation Volume: 20 Issue: 6 Pages: 869-874
  • DOI: 10.1107/s0909049513020827
  • Peer Reviewed
• [Journal Article] Change of dynamics of raft-model membrane induced by amyloid-beta protein binding (2013)
  • Author(s): Hirai, M., Kimura, R., Takeuchi, K., Sugiyama, M., Kasahara, K., Ohta, N., Farago, B., Stadler, A., Zaccai, G.
  • Journal Title: Eur.Phys.J.E.Soft Matter Volume: 36(7) Issue: 7 Pages: 9887-9887
  • DOI: 10.1140/epje/i2013-13074-3
  • Peer Reviewed
• [Presentation] 分子混雑環境におけるタンパク質構造の熱安定性と水和 (2016)
  • Author(s): 平井光博，味戸聡，高橋孝輔，佐藤笙喜
  • Organizer: PF研究会「徹底討論！小角散乱の魅力～基礎・応用・産業利用」
  • Place of Presentation: 高エネルギー加速器研究機構
  • Year and Date: 2016-03-30
  • Int'l Joint Research / Invited
• [Presentation] Crowding環 境下におけるタンパク質の熱安定性 (2016)
  • Author(s): 味戸聡志，平井光博
  • Organizer: 2015 年度 量子ビームサイエンスフェスタ
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-03-15
• [Presentation] DDSモデルとしてのタンパク質内包リポソームの研究 (2016)
  • Author(s): 高橋孝輔，平井光博
  • Organizer: 2015 年度 量子ビームサイエンスフェスタ
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2016-03-15
• [Presentation] 分子混雑環境下のタンパク質の構造，水和，熱安定性 (2015)
  • Author(s): 平井光博
  • Organizer: 日本中性子科学会第15 回年会
  • Place of Presentation: 和光市民文化センター
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] アミロイドタンパク質と相互作用における混合質膜の構造特性 (2015)
  • Author(s): 平井光博
  • Organizer: 低温研研究集会「生物の低温適応の分子機構」
  • Place of Presentation: 北海道大学
  • Year and Date: 2015-11-21
  • Invited
• [Presentation] CROWDING EFFECT ON PROTEIN STRUCTURE STABILITY CLARIFIED BY X-RAY AND NEUTRON SCATTERING (2015)
  • Author(s): M. Hirai, S. Sato, M. Sugiyama, N. Ohta, N. Shimizu, N. Igarashi, A. Martel, and L. Porcar
  • Organizer: 16TH INTERNATIONAL CONFERENCE ON SMALLANGLE SCATTERING
  • Place of Presentation: ベルリン工科大学
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] Interaction of amyloid beta protein with raft-model liposome (2015)
  • Author(s): S. Sato, M. Hirai, N. Ohta, N. Shimizu, and N. Igarashi
  • Organizer: 16TH INTERNATIONAL CONFERENCE ON SMALLANGLE SCATTERING
  • Place of Presentation: ベルリン工科大学
  • Year and Date: 2015-09-13
• [Presentation] Crowding環境におけるタンパク質の構造、水和、熱安定性 (2015)
  • Author(s): 平井光博
  • Organizer: 第３回Neutrons in Biology研究会
  • Place of Presentation: 日本原子力研究開発機構・原子力科学研究所
  • Year and Date: 2015-03-30 – 2015-03-31
  • Invited
• [Presentation] 生体膜とアミロイドbetaタンパク質との相互作用に於ける分子混雑の効果 (2015)
  • Author(s): 佐藤笙喜，平井光博
  • Organizer: 第3回物構研サイエンスフェスタ
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2015-03-17 – 2015-03-18
• [Presentation] 分子crowding環境下に於けるタンパク質の構造転移と水和 (2014)
  • Author(s): 平井光博
  • Organizer: 低温研研究集会「生物の低温適応の分子機構（２）」
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-12-26 – 2014-12-27
  • Invited
• [Presentation] Crowding 環境下でのタンパク質構造の熱安定性 (2014)
  • Author(s): Mitsuhiro Hirai, Shouki Sato, Masaaki Sugiyama, Noboru Ohota, Lionel Porcar, Anne Martel, Giuseppe Zaccai
  • Organizer: 第５２回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 時分割広角 X 線散乱によるラフトモデルリポソームとアミロイドベータタンパク質との相互作用に関する研究 (2014)
  • Author(s): Shoki Sato, Mitsuhiro Hirai, Noboru Ohta
  • Organizer: 第５２回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Structures of liposome encapsulating proteins under the osmotic pressure (2013)
  • Author(s): Ryota Kimura, Mitsuhiro Hirai
  • Organizer: The 51st Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kyoto
• [Presentation] Change of Dynamics of Raft-Model Membrane Induced by Amyloid-β Protein Binding (2013)
  • Author(s): Mitsuhiro Hirai, Ryota Kimura, Kazuki Takeuchi, Moboru Ohta, Bela Farago, Stadler Stadler, Giuseppe Zaccai
  • Organizer: The 51st Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kyoto
• [Presentation] Crowding effect on thermal transition of proteins clarified by SR-WAXS (2013)
  • Author(s): Kazuki Takeuchi, Mitsuhiro Hirai
  • Organizer: The 51st Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Kyoto
• [Presentation] Effect of crowded co-solutes on protein structure (2013)
  • Author(s): K. Takeuchi, M. Hirai, N. Ohta, N. Shimuzu, and N. Igarashi
  • Organizer: 4th International Symposium on Diffraction Structural Biology
  • Place of Presentation: Nagoya
• [Presentation] Structure and thermal stability of liposome entrapping proteins (2013)
  • Author(s): R. Kimura, M. Hirai, N. Ohta, N. Igarashi, and N. Shimuzu
  • Organizer: 4th International Symposium on Diffraction Structural Biology
  • Place of Presentation: Nagoya