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Study of functional structure of protein and biomembrane by the developement of resonant X-ray solution scattering in wide-energy range

Research Project

Project/Area Number 25440063
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionGunma University

Principal Investigator

Hirai Mitsuhiro  群馬大学, 大学院理工学府, 教授 (00189820)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsX線散乱 / タンパク質 / 生体膜 / 共鳴散乱 / X線 / 脂質膜 / 折畳み / 共鳴X線散乱 / 吸収端 / 溶液 / 複合体 / 広角散乱
Outline of Final Research Achievements

The use of anomalous dispersion of X-ray (resonant X-ray scattering) is expected to serve us a new insight on functional structures of proteins and membrane in solutions, especially of metalloproteins proteins containing metal atoms or clusters that are involved in a wide range of important biological processes. On the other hand, the recent development of the wide-angle X-ray scattering technique of proteins and membrane using third-generation synchrotron sources shows us that the X-ray solution scattering enables to observe a whole hierarchical structure ranging from ~0.2 nm to ~250 nm, namely, from the secondary structure to the quaternary one of proteins or from the alkylchain packing to the whole structure of lipid aggregate.
The aim of this study was the development of the new method for studying the internal structure of biological material in-situ by the combination of resonant and wide-angle scattering of X-ray.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report

Research Products

(22 results)

All 2016 2015 2014 2013

All Journal Article Presentation

  • [Journal Article] Effect of Protein-Encapsulation on Thermal Structural Stability of Liposome Composed of Glycosphingolipid/Cholesterol/Phospholipid2015

    • Author(s)
      Mitsuhiro Hirai, Shoki Sato, Ryota Kimura, Yoshihiko Hagiwara, Rika Kawai-Hirai, Noboru Ohta, Noriyuki Igarashi, and Nobutaka Shimizu
    • Journal Title

      J. Phys. Chem. B

      Volume: 119 Pages: 3398-3406

    • DOI

      10.1021/jp511534u

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Normalization of grazing-incidence small angle scattering of phospholipid alloy systems at the K absorption edge of phosphorous: A standard sample approach2014

    • Author(s)
      H.Okuda, T.Yamamoto,K.Takshita, M.Hirai, K.Senoo, H.Ogawa and Y.Kitajima
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 53 Issue: 5S1 Pages: 05FH02-05FH02

    • DOI

      10.7567/jjap.53.05fh02

    • NAID

      210000143840

    • ISSN
      0021-4922, 1347-4065
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conformational characterization of a protein complex involving intrinsically disordered protein by small-angle neutron scattering using the inverse contrast matching method: a case study of interaction between a-synuclein and PbaB tetramer as a model chaperone2014

    • Author(s)
      Masaaki Sugiyama
    • Journal Title

      J. Appl. Cryst

      Volume: 47 Pages: 430-435

    • DOI

      10.1107/s1600576713033475

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling2013

    • Author(s)
      Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N
    • Journal Title

      J. Synchrotron Rad.

      Volume: 20 Pages: 869-874

    • DOI

      10.1107/s0909049513020827

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Change of dynamics of raft-model membrane induced by amyloid-β protein binding2013

    • Author(s)
      Hirai, M., Kimura, R., Takeuchi, K., Sugiyama, M., Kasahara, K., Ohta, N., Farago, B., Stadler, A., Zaccai, G.
    • Journal Title

      Eur. Phys. J. E

      Volume: 36(7) Pages: 1-10

    • DOI

      10.1140/epje/i2013-13074-3

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 分子混雑環境におけるタンパク質構造の熱安定性と水和2016

    • Author(s)
      平井光博,味戸聡,高橋孝輔,佐藤笙喜
    • Organizer
      PF研究会「徹底討論!小角散乱の魅力~基礎・応用・産業利用」
    • Place of Presentation
      高エネルギー加速器研究機構
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Crowding環 境下におけるタンパク質の熱安定性2016

    • Author(s)
      味戸聡志,平井光博
    • Organizer
      2015 年度 量子ビームサイエンスフェスタ
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] DDSモデルとしてのタンパク質内包リポソームの研究2016

    • Author(s)
      高橋孝輔,平井光博
    • Organizer
      2015 年度 量子ビームサイエンスフェスタ
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-03-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分子混雑環境下のタンパク質の構造,水和,熱安定性2015

    • Author(s)
      平井光博
    • Organizer
      日本中性子科学会第15 回年会
    • Place of Presentation
      和光市民文化センター
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アミロイドタンパク質と相互作用における混合質膜の構造特性2015

    • Author(s)
      平井光博
    • Organizer
      低温研研究集会「生物の低温適応の分子機構」
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-11-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] CROWDING EFFECT ON PROTEIN STRUCTURE STABILITY CLARIFIED BY X-RAY AND NEUTRON SCATTERING2015

    • Author(s)
      M. Hirai, S. Sato, M. Sugiyama, N. Ohta, N. Shimizu, N. Igarashi, A. Martel, and L. Porcar
    • Organizer
      16TH INTERNATIONAL CONFERENCE ON SMALLANGLE SCATTERING
    • Place of Presentation
      ベルリン工科大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Interaction of amyloid beta protein with raft-model liposome2015

    • Author(s)
      S. Sato, M. Hirai, N. Ohta, N. Shimizu, and N. Igarashi
    • Organizer
      16TH INTERNATIONAL CONFERENCE ON SMALLANGLE SCATTERING
    • Place of Presentation
      ベルリン工科大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Crowding環境におけるタンパク質の構造、水和、熱安定性2015

    • Author(s)
      平井光博
    • Organizer
      第3回Neutrons in Biology研究会
    • Place of Presentation
      日本原子力研究開発機構・原子力科学研究所
    • Year and Date
      2015-03-30 – 2015-03-31
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 生体膜とアミロイドbetaタンパク質との相互作用に於ける分子混雑の効果2015

    • Author(s)
      佐藤笙喜,平井光博
    • Organizer
      第3回物構研サイエンスフェスタ
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-03-17 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 分子crowding環境下に於けるタンパク質の構造転移と水和2014

    • Author(s)
      平井光博
    • Organizer
      低温研研究集会「生物の低温適応の分子機構(2)」
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-12-26 – 2014-12-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Crowding 環境下でのタンパク質構造の熱安定性2014

    • Author(s)
      Mitsuhiro Hirai, Shouki Sato, Masaaki Sugiyama, Noboru Ohota, Lionel Porcar, Anne Martel, Giuseppe Zaccai
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 時分割広角 X 線散乱によるラフトモデルリポソームとアミロイドベータタンパク質との相互作用に関する研究2014

    • Author(s)
      Shoki Sato, Mitsuhiro Hirai, Noboru Ohta
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Structures of liposome encapsulating proteins under the osmotic pressure2013

    • Author(s)
      Ryota Kimura, Mitsuhiro Hirai
    • Organizer
      The 51st Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Research-status Report
  • [Presentation] Change of Dynamics of Raft-Model Membrane Induced by Amyloid-β Protein Binding2013

    • Author(s)
      Mitsuhiro Hirai, Ryota Kimura, Kazuki Takeuchi, Moboru Ohta, Bela Farago, Stadler Stadler, Giuseppe Zaccai
    • Organizer
      The 51st Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Research-status Report
  • [Presentation] Crowding effect on thermal transition of proteins clarified by SR-WAXS2013

    • Author(s)
      Kazuki Takeuchi, Mitsuhiro Hirai
    • Organizer
      The 51st Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Research-status Report
  • [Presentation] Effect of crowded co-solutes on protein structure2013

    • Author(s)
      K. Takeuchi, M. Hirai, N. Ohta, N. Shimuzu, and N. Igarashi
    • Organizer
      4th International Symposium on Diffraction Structural Biology
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Research-status Report
  • [Presentation] Structure and thermal stability of liposome entrapping proteins2013

    • Author(s)
      R. Kimura, M. Hirai, N. Ohta, N. Igarashi, and N. Shimuzu
    • Organizer
      4th International Symposium on Diffraction Structural Biology
    • Place of Presentation
      Nagoya
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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