Study of functional structure of protein and biomembrane by the developement of resonant X-ray solution scattering in wide-energy range
| Project/Area Number |
25440063
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | X線散乱 / タンパク質 / 生体膜 / 共鳴散乱 / X線 / 脂質膜 / 折畳み / 共鳴X線散乱 / 吸収端 / 溶液 / 複合体 / 広角散乱 |
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| Outline of Final Research Achievements |
The use of anomalous dispersion of X-ray (resonant X-ray scattering) is expected to serve us a new insight on functional structures of proteins and membrane in solutions, especially of metalloproteins proteins containing metal atoms or clusters that are involved in a wide range of important biological processes. On the other hand, the recent development of the wide-angle X-ray scattering technique of proteins and membrane using third-generation synchrotron sources shows us that the X-ray solution scattering enables to observe a whole hierarchical structure ranging from ~0.2 nm to ~250 nm, namely, from the secondary structure to the quaternary one of proteins or from the alkylchain packing to the whole structure of lipid aggregate.
The aim of this study was the development of the new method for studying the internal structure of biological material in-situ by the combination of resonant and wide-angle scattering of X-ray.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Structure of liposome encapsulating proteins characterized by X-ray scattering and shell-modeling2013
Author(s)
Hirai, M., Kimura, R., Takeuchi, K., Hagiwara, Y., Kawai-Hirai, R., Ohta, N., Igarashi, N. and Shimizu, N
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Journal Title
J. Synchrotron Rad.
Volume: 20
Pages: 869-874
DOI
Related Report
Peer Reviewed
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[Journal Article] Change of dynamics of raft-model membrane induced by amyloid-β protein binding2013
Author(s)
Hirai, M., Kimura, R., Takeuchi, K., Sugiyama, M., Kasahara, K., Ohta, N., Farago, B., Stadler, A., Zaccai, G.
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Journal Title
Eur. Phys. J. E
Volume: 36(7)
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Presentation] Crowding 環境下でのタンパク質構造の熱安定性2014
Author(s)
Mitsuhiro Hirai, Shouki Sato, Masaaki Sugiyama, Noboru Ohota, Lionel Porcar, Anne Martel, Giuseppe Zaccai
Organizer
第５２回日本生物物理学会年会
Place of Presentation
札幌コンベンションセンター
Year and Date
2014-09-25 – 2014-09-27
Related Report
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