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Novel methods to inhibit neurofibrillary tangles of tau protein: functional modifications of peptidyl prolyl isomerases

Research Project

Project/Area Number 25440064
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biophysics
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Ikura Teikichi  東京医科歯科大学, 難治疾患研究所, 准教授 (50251393)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsタウタンパク質 / プロリン異性化酵素 / 神経原線維変化 / 機能改変 / Pin1 / プロリン異性化 / 凝集化 / アルツハイマー病 / 脱リン酸化 / PP2A / リン酸化 / プロリン異性化活性 / 凝集
Outline of Final Research Achievements

The Alzheimer's disease-related protein, tau, aggregates into neurofibrillary tangles (NFT) when it is hyperphosphorylated. In this research project, I aimed to inhibit formation of TNF, stabilize microtubule, and furthermore prevent the Alzheimer's disease. In my strategy, I focused on the specific interactions between tau protein and peptidyl-prolyl isomerases (PPIases), Pin1 and FKBP12: PPIases restored the function of tau protein by presumably catalyzing isomerization of a specific pS/T-P or X-P motif. In this study, I introduced the comprehensive mutations at the active site of the PPIases and finally succeeded in discovery of several mutant proteins with higher activity than wild-type protein. Moreover, I tackled a functional change of Pin1 in which Pin1 was converted from a PPIase to a protease, and finally succeeded in creating a novel protease with the specificity to Proline residue including pS/pT-P motif of tau protein.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] Peptidyl-prolyl isomerase activity of FK506 binding protein 12 prevents tau peptide from aggregating.2013

    • Author(s)
      Ikura, T., Ito, N.
    • Journal Title

      Protein Eng. Des. Sel.

      Volume: 26 Issue: 9 Pages: 539-546

    • DOI

      10.1093/protein/gzt033

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Pin1由来のタンパク質分解酵素の触媒機構の解析2016

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] 1アミノ酸置換によるプロリン異性化酵素からタンパク質分解酵素への機能転換2015

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Pin1はタウペプチドのpT231-P232の異性化を触媒しないが、凝集を抑制する2015

    • Author(s)
      伊倉貞吉, 栃尾尚哉, 川嵜亮佑, 楯真一, 伊藤暢聡
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      徳島・あわぎんホール
    • Year and Date
      2015-06-24 – 2015-06-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Pin1 のプロリン異性化活性とタウタンパク質に対する凝集抑制活性との関係2014

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌・札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] How do peptidyl-prolyl isomerases rescue tau protein from aggregating?2014

    • Author(s)
      Teikichi Ikura and Nobutoshi Ito
    • Organizer
      IUPAB2014
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2014-08-03 – 2014-08-07
    • Related Report
      2014 Research-status Report
  • [Presentation] タウタンパク質の脱リン酸化におけるPin1とPP2Aの協同性の分子機構2014

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜・ワークピア横浜/横浜産貿ホール マリネリア
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] プロリン異性化反応がタウタンパク質の凝集を阻害する2013

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館
    • Related Report
      2013 Research-status Report
  • [Presentation] タウタンパク質に対するPin1のプロリン異性化活性を測定するための新しい方法2013

    • Author(s)
      伊倉貞吉, 伊藤暢聡
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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