| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We over-expressed, purified and crystallized a C-terminal 564 residues of gp34, proximal long tail fiber, of bacteriophage T4. A determined crystal structure of gp34C774-1289 is trimeric fibrous protein which is 18nm long. In detail, there are three-stranded beta-helix and beta-prism domains which are found in other phage tail protein. Thus we conclude that the phage tail proteins are evolved with utilizing the “tail parts domains” to obtain their ability for their infection.
We also purified gp38 as a complex with C-terminal portion of gp37 by co-expression system.
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