Structural biology of phage long tail fiber protein and host recognition
| Project/Area Number |
25440066
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Kanamaru Shuji 東京工業大学, 生命理工学院, 助教 (50376951)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バクテリオファージ / ウイルス / 構造蛋白質 / 繊維状蛋白質 / X線結晶構造解析 / 宿主認識 / ウイルス蛋白質 / 構造解析 / 結晶構造解析 / ファージ / 蛋白質複合体 / 尾繊維 / 大腸菌 / 分子内シャペロン |
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| Outline of Final Research Achievements |
We over-expressed, purified and crystallized a C-terminal 564 residues of gp34, proximal long tail fiber, of bacteriophage T4. A determined crystal structure of gp34C774-1289 is trimeric fibrous protein which is 18nm long. In detail, there are three-stranded beta-helix and beta-prism domains which are found in other phage tail protein. Thus we conclude that the phage tail proteins are evolved with utilizing the “tail parts domains” to obtain their ability for their infection.
We also purified gp38 as a complex with C-terminal portion of gp37 by co-expression system.
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] NADH oxidase and alkyl hydroperoxide reductase subunit C (peroxiredoxin) from Amphibacillus xylanus form an oligomeric assembly.2015
Author(s)
Toshiaki Arai, Shinya Kimata, Daichi Mochizuki, Keita Hara, Tamotsu Zako, Masafumi Odaka, Masafumi Yohda, Fumio Arisaka, Shuji Kanamaru, Takashi Matsumoto, Shunsuke Yajima, Junichi Sato, Shinji Kawasaki, Youichi Niimura
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Journal Title
FEBS Open Bio.
Volume: 5
Pages: 124-131
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Crystallization of the carboxy-terminal region of the bacteriophage T4 proximal long tail fibre protein gp342014
Author(s)
Granell, M., Namura, M., Alvira, S., Garcia-Doval, C., Singh, A. K., Gutsche, I., van Raaij, M. J. & Kanamaru, S.
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Journal Title
Acta Crystallogr F Struct Biol Commun
Volume: 70
Pages: 970-975
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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