Development and application of the experimental platform for the single-molecule analysis of the lipid-effects on the ion channel function
| Project/Area Number |
25440067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 生体膜 / 1分子計測 / チャネル / 脂質 / 電気生理 / １分子計測 |
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| Outline of Final Research Achievements |
Based on the principle of formation of the contact bilayer between water-in-oil droplets, we developed the platform for the artificial membrane experiments to analyze quantitatively the "lipid-effects" on the function of the membrane proteins. We prepared a variety of lipid bilayer membranes having different lipid composition including asymmetric membranes on the platform. We reconstituted the KcsA potassium channel into these membranes and succeeded in detecting the lipid-dependency in the activity of the channel at single-molecule level.
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Report
(4 results)
Research Products
(21 results)
