| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
There are many examples of proteins interacting with each other while maintaining their specific three-dimensional structures, and leading to aggregation. Such aggregation is also associated with many kinds of diseases. It is also one of the causes that hinder detailed spectroscopic analyses such as NMR. The purpose of this study is to study the physical mechanism by which such aggregation occurs spontaneously and to develop chemical modification methods that inhibit it. I have targeted VanX, an enzyme involved in vancomycin resistance in Enterococci. The tertiary structure as a dimer was analyzed mainly by NMR. As a result, it was shown that VanX aggregates to tetramers as the concentration increases. It was also found that the degree of aggregation changes depending on the solvent pH.
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