| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Amyloid fibrils are a form of protein aggregates associated with numerous diseases. One of the most unique and essential properties is their ability to self-propagate, which leads to the explosive amplification of amyloid fibrils and then the onset of the diseases; however, much remains to be elucidated regarding its molecular details how and when the propagating nuclei initially emerge.
In this study, we have trapped and characterized structural properties of prefibrillar intermediate species of insulin and an insulin-derived short peptide, and as for insulin, we have clarified time-dependent formation and evolution of the prefibrillar intermediates by time-resolved small angle X-ray scattering measurement. We also performed time-resolved NIR spectral measurement to clarify more details of the water molecular system dynamics during the nucleation.
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