Elucidation of the structure and function of a photoactivated adenylyl cyclase from a sulfer bacterium
Project/Area Number |
25440072
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Toho University |
Principal Investigator |
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 光センサー / フラビン / アデニル酸シクラーゼ / cAMP / 光生物学 / 光受容体 |
Outline of Final Research Achievements |
This research is aimed at elucidating the activation mechanism of photoactivated adenylyl cyclase (PAC). We found a new homolog of PAC in the genome sequence of a cyanobacterium Oscillatoria acuminata and demonstrated that the homolog (OaPAC) can be easily expressed and purified from E. coli as soluble form. Using the purified protein, we successfully determined the photochemical and biochemical properties of the protein, and solved its crystal structure under collaboration with structure biologists.
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Report
(4 results)
Research Products
(17 results)