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Elucidation of the kinase module to control the endoplasmic reticulum stress response

Research Project

Project/Area Number 25440078
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Mizuno Tomoaki  筑波大学, 医学医療系, 助教 (80529032)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords小胞体ストレス / MAPキナーゼ / AMP活性化キナーゼ / AMPK / キナーゼ / ホスファターゼ
Outline of Final Research Achievements

Accumulation of unfolded proteins in the lumen of the endoplasmic reticulum (ER) causes ER stress. Snf1, the Saccharomyces cerevisiae ortholog of AMP-activated protein kinase (AMPK), plays a crucial role in the response to various environmental stresses. However, the role of Snf1 in ER stress response remains poorly understood. In this study, we characterize Snf1 as a negative regulator of Hog1 MAPK in ER stress response. The snf1 mutants showed the ER stress resistant phenotype. Activated Hog1 levels were increased by snf1 mutation. Ssk1, a specific activator of MAPKKK functioning upstream of Hog1, was induced by ER stress and its induction was inhibited in a manner dependent on Snf1 activity. Furthermore, we show that the SSK1 promoter is important for Snf1-modulated regulation of Ssk1 expression. Our data suggest that Snf1 downregulates ER stress response signal mediated by Hog1 through negatively regulating expression of its specific activator Ssk1 at the transcriptional level.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] The Saccharomyces cerevisiae AMPK, Snf1, Negatively Regulates the Hog1 MAPK Pathway in ER Stress Response.2015

    • Author(s)
      Mizuno T, Masuda Y, Irie K
    • Journal Title

      PLOS Genetics

      Volume: 11 Issue: 9 Pages: e1005491-e1005491

    • DOI

      10.1371/journal.pgen.1005491

    • NAID

      120007136050

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Regulation of endoplasmic reticulum stress response by the budding yeast AMPK ortholog Snf12014

    • Author(s)
      Yuto Masuda
    • Organizer
      第37回分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 出芽酵母AMPKオルソログSnf1の小胞体ストレス応答における機能解析2014

    • Author(s)
      増田勇人
    • Organizer
      酵母遺伝学フォーラム第47回研究報告会
    • Place of Presentation
      東京大学
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Genetic interaction between KIN1/KIN2 and UBA42013

    • Author(s)
      Yuto Masuda
    • Organizer
      12th NTU Kyoto Tsukuba joint mini-symposium
    • Place of Presentation
      台湾
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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