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Application of TOF-SIMS imaging to C. elgans

Research Project

Project/Area Number 25440082
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cell biology
Research InstitutionKanagawa University of Human Services (2015)
Hamamatsu University School of Medicine (2013-2014)

Principal Investigator

Kimura Yoshishige  神奈川県立保健福祉大学, 保健福祉学部, 准教授 (90274703)

Co-Investigator(Renkei-kenkyūsha) SETOU Mitsutoshi  浜松医科大学, 医学部, 教授 (20302664)
Research Collaborator HAMEED Saira  
GOTO Kensuke  
ISHIZAKI Itsuko  
SANADA Noriaki  
SUZUKI Mayumi  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords質量分析イメージング法 / TOF-SIMS / 線虫 / メタボローム / イメージングマススペクトロメトリー / メタボローム解析
Outline of Final Research Achievements

Imaging mass spectrometry (IMS) is a two-dimensional mass spectrometry to visualize the spatial distribution of biomolecules, which does not need either separation or purification of target molecules. In this study, our goal is to reveal the cellar distribution of biomolecules of C. elegans (a commonly used model organism for the life science) by application of IMS approach. Since C. elegans is a small animal, we have used TRIFT V, TOF-SIMS (Time-of-Flight Secondary Mass Spectrometry) system (ULVAC-PHI, Inc), which enables us to obtain nanoscale microstructure. By comparison of several sample preparation methods, the frozen sections of C. elegans fixed by paraformaldehyde (PFA) were most suitable for TOF-SIMS analysis. By sputtering of Ar gas cluster ion beam (Ar-GCIB), the sensitivity to fatty acids (e.g. stearic acid (SA), oleic acid (OA), and eicosapentaenoic acid(EPA)) was significantly enhanced, and high resolution images of biomolecules were successfully acquired.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2013

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results)

  • [Journal Article] Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry.2015

    • Author(s)
      Hameed S, Ikegami K, Sugiyama E, Matsushita S, Kimura Y, Hayasaka T, Sugiura Y, Masaki N, Waki M, Ohta I, Hossen MA, Setou M
    • Journal Title

      Anal Bioanal Chem

      Volume: 407 Issue: 25 Pages: 7589-7602

    • DOI

      10.1007/s00216-015-8932-7

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Intraflagellar transport in sensory cilia is tuned by environmental stimuli by tubulin polyglutamylation.2013

    • Author(s)
      Kimura, Y. Konno, A., Tsutsumi, K., Hameed, S., Ikegami, K., Setou, M.
    • Organizer
      北米細胞生物学会
    • Place of Presentation
      ニューオーリンズ(米国)
    • Related Report
      2013 Research-status Report
  • [Presentation] Environmental stimulus-responsive behavioral plasticity is regulated by intraflagellar transport of sensory cilia.2013

    • Author(s)
      Kimura, Y., Tsutsumi, K., Konno, A., Ikegami, K., Hameed, S, Kaplan, O.I., Teramoto,T., Fujiwara, M., Kunitomo, H., Ishihara, K., Iino, Y., Blacque, O.E., Setou, M.
    • Organizer
      FASEB Meeting ----Biology of Cilia and Flagella
    • Place of Presentation
      ナイアガラフォールズ(米国)
    • Related Report
      2013 Research-status Report
  • [Presentation] Starvation-responsive behavioral plasticity is tuned by tubulin polyglutamylation in sensory cilia2013

    • Author(s)
      Kimura, Y., Tsutsumi, K., Konno, A., Ikegami, K., Hameed, S, Kaplan, O.I., Teramoto,T., Fujiwara, M., Kunitomo, H., Ishihara, K., Iino, Y., Blacque, O.E., Setou, M
    • Organizer
      国際線虫ミーティング
    • Place of Presentation
      ロサンゼルス(米国)
    • Related Report
      2013 Research-status Report
  • [Presentation] Environmental responsiveness in intraflagellar transport is regulated by tubulin polyglutamylation.2013

    • Author(s)
      木村芳滋, 紺野在, Saira Hameed, 瀬藤光利
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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