RNA recognition by mRNA export receptor subunit p15

• Project/Area Number: 25440083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: Jun Katahira 大阪大学, 医学(系)研究科(研究院), 准教授 (30263312)
• Research Collaborator: Dimitrova Lyudmila ; Hurt Ed ; Aibara Shintaro ; Stewart Murray
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 核－細胞質間輸送 / 遺伝子発現制御 / 遺伝子発現調節 / 国際情報交換 / RNA

Outline of Final Research Achievements

The aim of this study is elucidation of the molecular mechanism of “cargo” mRNA recognition by the nuclear export receptor Tap-p15 heterodimer. We have shown that three distinct domains of Tap including RRM-LRR-NTF2L heterodimerized with p15 are involved in the cargo mRNA recognition. The results suggest that the Tap-p15 heterodimer recognizes bulk cellular mRNAs and viral constitutive transport element (CTE)-containing mRNA by differentially using these distinct RNA binding domains.

Research Products

• [Int'l Joint Research] ハイデルベルグ大学(ドイツ)
• [Int'l Joint Research] MRC(英国)
• [Journal Article] The principal mRNA nuclear export factor NXF1:NXT1 forms a symmetric binding platform that facilitates export of retroviral CTE-RNA. (2015)
  • Author(s): Aibara, S., Katahira, J., Valkov, E. Stewart, M.
  • Journal Title: Nucleic Acids Res. Volume: 43 Issue: 3 Pages: 1883-1893
  • DOI: 10.1093/nar/gkv032
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export. (2015)
  • Author(s): Katahira, J., Dimitrova, L., Imai, Y., Hurt, E.
  • Journal Title: Nucleic Acids Res. Volume: 43 Issue: 3 Pages: 1894-1904
  • DOI: 10.1093/nar/gkv039
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nuclear export of messenger RNA. (2015)
  • Author(s): Katahira, J.
  • Journal Title: Genes (Basel) Volume: 6 Issue: 2 Pages: 163-184
  • DOI: 10.3390/genes6020163
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] mRNA核外輸送複合体の形成機構 (2015)
  • Author(s): 片平じゅん
  • Journal Title: 生化学 Volume: 87 Pages: 75-81
• [Journal Article] Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I (2013)
  • Author(s): Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y
  • Journal Title: Nucleic Acids Res Volume: 41(14) Issue: 14 Pages: 7060-72
  • DOI: 10.1093/nar/gkt414
  • Peer Reviewed
• [Presentation] TapのNTF2Lドメインは積み荷mRNAの認識と核外輸送に重要な役割を果たす (2015)
  • Author(s): 片平じゅん
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-02
• [Presentation] 輸送受容体Tap-p15ヘテロダイマーによる積み荷RNA認識機構 (2013)
  • Author(s): 片平じゅん
  • Organizer: 日本分子生物学会 ワークショップ
  • Place of Presentation: 神戸
• [Presentation] ヒトTREX複合体構成因子Thoc5はCFImのリクルートメントを介してポリアデニレーション部位選択に関与する (2013)
  • Author(s): 片平じゅん、奥崎大介、井上仁美、米田悦啓、前原一満、大川恭行
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 箱根